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Thermotolerance of staphylococcus aureus after sublethal heat shock
Other Title(s)
المقاومة الحرارية لخلايا بكتيريا المكورات العنقودية الذهبية عقب التعرض للصدمة الحرارية دون المميته
Joint Authors
Khallaf, Muhammad Faraj
Muhammad, Wafa Munji
al-Bayyumi, Midhat Muhammad
Hasan, Amal Ahmad
Source
Arab Universities Journal of Agricultural Sciences
Issue
Vol. 27, Issue 1 (s) (31 Mar. 2019), pp.467-477, 11 p.
Publisher
Ain Shams University Faculty of Agriculture
Publication Date
2019-03-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Topics
- Electron microscopes
- Thermal properties
- Molecular weights
- Therapy
- Food Safety
- Staphylococcus
- ًElectric shocks
- Heat shock proteins
Abstract EN
The effect of prior heat shock on the thermotolerance of Staphylococcus aureus in broth culture and induction of heat shock proteins was investigated.
S.
aureus cell wall was, also, examined using Scanning Electron Microscope (SEM).
Specific bacteria were grown at 37°C the optimum temperature (control), sublethally heated at 47, 52, 57 and 60°C for various times, and heat treated at 68°C were done.
The estimated average of the D68-values for the control was 4.83 min while for heat shocked treatments ranged from 5.43 to 10.23 min.
The current results, also, indicated that 8 - 16 new bands were induced by applying heat shock treatments with molecular weights ranged from 9 to 135 KDa.
The induced heat shock proteins further confirmed the increased the thermotolerance.
Moreover, selected heat shock treatments caused severe destruction in cell wall (i.
e.
, rupture, irregular and leakage of cell contents), while heat shocked cells after incubating at 37°C for overnight in enriched medium became similar to that of the one's normal.
The enhanced heat resistance of S.
aureus should be thought-about in cause of planning effective thermal processes to confirm the microbiological food safety.
American Psychological Association (APA)
Muhammad, Wafa Munji& Khallaf, Muhammad Faraj& Hasan, Amal Ahmad& al-Bayyumi, Midhat Muhammad. 2019. Thermotolerance of staphylococcus aureus after sublethal heat shock. Arab Universities Journal of Agricultural Sciences،Vol. 27, no. 1 (s), pp.467-477.
https://search.emarefa.net/detail/BIM-999833
Modern Language Association (MLA)
Muhammad, Wafa Munji…[et al.]. Thermotolerance of staphylococcus aureus after sublethal heat shock. Arab Universities Journal of Agricultural Sciences Vol. 27, no. 1 (s) (2019), pp.467-477.
https://search.emarefa.net/detail/BIM-999833
American Medical Association (AMA)
Muhammad, Wafa Munji& Khallaf, Muhammad Faraj& Hasan, Amal Ahmad& al-Bayyumi, Midhat Muhammad. Thermotolerance of staphylococcus aureus after sublethal heat shock. Arab Universities Journal of Agricultural Sciences. 2019. Vol. 27, no. 1 (s), pp.467-477.
https://search.emarefa.net/detail/BIM-999833
Data Type
Journal Articles
Language
English
Notes
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Record ID
BIM-999833