Biological control of soil-borne fungal pathogens

Other Title(s)

المقاومة الحيوية للفطريات الممرضة بالتربة

Joint Authors

Afifi, Muhammad Abd al-Aziz
Mitri, Imad Anis
Madkur, Majdi Ahmad
Ismail, Ismail Muhammad Kamil

Source

Arab Universities Journal of Agricultural Sciences

Publisher

Ain Shams University Faculty of Agriculture

Publication Date

2019-03-31

Country of Publication

Egypt

No. of Pages

12

Main Subjects

Agriculture

Topics

• Activities
• Biological control
• Plant diseases
• Pathogenic fungi
• Bacillus cereus
• Rhizoctonia solani

English Abstract

Seventeen bacterial isolates have been successfully isolated and purified from soil rhizosphere samples collected from different agricultural areas Buhayra, Sharqia and Alqilyubia of Egypt, and screened for production of chitinase enzymes had the highest chitinolytic activities amongst those investigated.

Isolate S3-C and S1-C were Identified by biochemical, physiological tests and 16s rRNA gene primer as Bacillus cereus S3C, Gene Bank NCBI accession MK185696 and Bacillus cereus S1C accession MK185697.

The production of chitinase by B.

cereus S3C and Bacillus cereus S1C was optimized using colloidal chitin medium amended with 1% colloidal chitin at 30°C after five days of incubation.

B.

cereus S3C had potential for cell wall lysis of many phytopathogenic fungi tested such as Fusarium oxysporum and Rhizoctonia solani Potato phytopathogenic fungi by in vitro antagonistic test.

The addition of B.

cereus S3C chitinase was more effective than that of B.

cereus S1C in increasing the resistance of Potato plants infected with various Soil- borne phytopathogenic fungi.

Data Type

Conference Papers

Record ID

BIM-999858

• APA
• MLA
• AMA