Isolation, identification and potential biological control of some rhizobacteria against meloidogyne incognita

Abstract EN

The plant-parasitic nematodes seriously effect on the growth of many crops and are responsible for agricultural losses worldwide.

The losses range from 8 to 20% on major crops around the world.

Root-knot nematode Meloidogyne spp.

infect and damage a wide range of important crops particularly vegetables in tropical and subtropical countries.

The main way for controlling of the plant parasitic nematodes is the use of chemical nematicides.

Although the nematicides are effective quickly, they are usually expensive and not available and also cause a lot of risk to humans and inflict injury to the environment.

Due to the environmental hazards associated with their application, identifying alternatives for nematode control and developing effective and safe application techniques is both urgent strategies for alleviating the nematode induced damage.

The biological controls are alternatives eco-friendly agricultural systems and safer for environments and humans and cheaper than chemical control
This study was conducted to isolate and estimate of the potential some rhizobacteria from roots in agriculture soil against root-knot nematode, Meloidogyne incognita J2 under in vitro condition.

Nine rhizobacteria were isolated from soil (R1 to R9).

The best mortality was recorded by isolate no.

R6 (77.55% ) followed by isolated no.

R2 (75.59% ) followed by isolate no.

R7 (71.43% ) as compared with 0% in case of control (water only) after 72h exposure periods.

R6, R2, and R7 were identified as Lysinibacillus sphaericus, Bacillus pumilus and Pseudomonas flourescens, respectively based on the analysis of the 16S rRNA gene sequence.

The partial 16S rRNA gene sequence of these bacterial isolates were deposited in GenBank under accession number MF000302, MF000303 and MF000304 for the previous mentioned bacterial species.

The main goals of this study were to isolate some native rhizobacteria and evaluate their efficiency as eco-friendly control alternatives for controlling root- knot nematode M.

incognita under laboratory conditions.