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In vitro callus induction and shoot regeneration potentials in some snake melon accessions collected from different regions in Egypt
Other Title(s)
كفاءة تكوين الكالوس و تجديد التبرعم الخضري معمليا في بعض سالالات القثاء المجمعة من مناطق مختلفة في مصر
Joint Authors
Ulwan, Muhammad Wasfi Muhammad
Abd al-Salam, Muhammad Muhammad
Muhammad, Fuad Hasan
Abu Zayd, Amiru Ahmad
Abd al-Hamid, Khalid al-Sayyid
Source
Arab Universities Journal of Agricultural Sciences
Issue
Vol. 27, Issue 4 (30 Sep. 2019), pp.2223-2233, 11 p.
Publisher
Ain Shams University Faculty of Agriculture
Publication Date
2019-09-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Topics
Abstract EN
Several snake melon genotypes are grown in different locations in Egypt.
However, the relationships and the degree of relatness among these genotypes are not well documented.
This study was carried out with the aim to classify different Egyptian snake melon genotypes based on the in vitro callus induction and shoot regeneration potential.
Nine snake melon accessions (acc.
) were collected from different regions in Egypt, including acc.3 (Damietta), acc.7 (Bany Swief), acc.8 (Fayoum), acc.9 (Giza), acc.11 (Menia), acc.14 (Sohag), acc.
15 (Behaira-Wady Elnatron), acc.
17 (Ismailia) and acc.
18 (Behaira-Badr Center).
Twenty seeds from each accession were sterilized and cultured in vitro on MS medium basal salt and vitamins for 4 weeks.
Segments (4x4mm each) from cotyledon were used as explants and cultured on MS medium amended with 2.0 mg/L TDZ for callus induction.
The formed callus was sub cultured onto MS medium amended with 3.0 mg/L BA+4ml Hyaluronic acid for shoot regeneration.
Results indicated that the degree of callus formation was different among the different accessions.
Based on callus growth potential measured as fresh weight, snake melon accessions could be ranked as: acc.9> acc.18> acc.3= acc.11> acc.17> acc.15> acc.8= acc.14> acc.7.
Significant differences among the accessions were also observed for their shoot regeneration potential from callus.
The highest number of shoots per explant was recorded in acc.
18 (ave.12.6 shoots), followed by acc.9, 11, and 7 which produced an average of 10.8, 10.4, and 9.8 shoots, respectively.
Results suggested that snake melon genotypes with high callus induction had also high regeneration capacity.
In addition, these accessions could have different genetic background, which might help in future breeding programs to improve plant and agronomic traits.
The current in vitro callus induction and shoot regeneration technique in snake melon will also aid in future effort for germplasm preservation of accessions with unique characteristics.
American Psychological Association (APA)
Muhammad, Fuad Hasan& Abu Zayd, Amiru Ahmad& Ulwan, Muhammad Wasfi Muhammad& Abd al-Hamid, Khalid al-Sayyid& Abd al-Salam, Muhammad Muhammad. 2019. In vitro callus induction and shoot regeneration potentials in some snake melon accessions collected from different regions in Egypt. Arab Universities Journal of Agricultural Sciences،Vol. 27, no. 4, pp.2223-2233.
https://search.emarefa.net/detail/BIM-999928
Modern Language Association (MLA)
Muhammad, Fuad Hasan…[et al.]. In vitro callus induction and shoot regeneration potentials in some snake melon accessions collected from different regions in Egypt. Arab Universities Journal of Agricultural Sciences Vol. 27, no. 4 (2019), pp.2223-2233.
https://search.emarefa.net/detail/BIM-999928
American Medical Association (AMA)
Muhammad, Fuad Hasan& Abu Zayd, Amiru Ahmad& Ulwan, Muhammad Wasfi Muhammad& Abd al-Hamid, Khalid al-Sayyid& Abd al-Salam, Muhammad Muhammad. In vitro callus induction and shoot regeneration potentials in some snake melon accessions collected from different regions in Egypt. Arab Universities Journal of Agricultural Sciences. 2019. Vol. 27, no. 4, pp.2223-2233.
https://search.emarefa.net/detail/BIM-999928
Data Type
Journal Articles
Language
English
Notes
-
Record ID
BIM-999928