A Novel Bone Morphogenetic Protein 2 Mutant Mouse, nBmp2NLStm, Displays Impaired Intracellular Ca2+ Handling in Skeletal Muscle
المؤلفون المشاركون
Bridgewater, Laura C.
Mayo, Jaime L.
Evanson, Bradley G.
Whitt, Megan E.
Dean, Spencer A.
Yates, Joshua D.
Holden, Devin N.
Schmidt, Alina D.
Fox, Christopher L.
Dhunghel, Saroj
Steed, Kevin S.
Adam, Michael M.
Nichols, Caitlin A.
Loganathan, Sampath K.
Barrow, Jeffery R.
Hancock, Chad R.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-11-28
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
We recently reported a novel form of BMP2, designated nBMP2, which is translated from an alternative downstream start codon and is localized to the nucleus rather than secreted from the cell.
To examine the function of nBMP2 in the nucleus, we engineered a gene-targeted mutant mouse model (nBmp2NLStm) in which nBMP2 cannot be translocated to the nucleus.
Immunohistochemistry demonstrated the presence of nBMP2 staining in the myonuclei of wild type but not mutant skeletal muscle.
The nBmp2NLStm mouse exhibits altered function of skeletal muscle as demonstrated by a significant increase in the time required for relaxation following a stimulated twitch contraction.
Force frequency analysis showed elevated force production in mutant muscles compared to controls from 10 to 60 Hz stimulation frequency, consistent with the mutant muscle’s reduced ability to relax between rapidly stimulated contractions.
Muscle relaxation after contraction is mediated by the active transport of Ca2+ from the cytoplasm to the sarcoplasmic reticulum by sarco/endoplasmic reticulum Ca2+ ATPase (SERCA), and enzyme activity assays revealed that SERCA activity in skeletal muscle from nBmp2NLStm mice was reduced to approximately 80% of wild type.
These results suggest that nBMP2 plays a role in the establishment or maintenance of intracellular Ca2+ transport pathways in skeletal muscle.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bridgewater, Laura C.& Mayo, Jaime L.& Evanson, Bradley G.& Whitt, Megan E.& Dean, Spencer A.& Yates, Joshua D.…[et al.]. 2013. A Novel Bone Morphogenetic Protein 2 Mutant Mouse, nBmp2NLStm, Displays Impaired Intracellular Ca2+ Handling in Skeletal Muscle. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1003284
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bridgewater, Laura C.…[et al.]. A Novel Bone Morphogenetic Protein 2 Mutant Mouse, nBmp2NLStm, Displays Impaired Intracellular Ca2+ Handling in Skeletal Muscle. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1003284
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bridgewater, Laura C.& Mayo, Jaime L.& Evanson, Bradley G.& Whitt, Megan E.& Dean, Spencer A.& Yates, Joshua D.…[et al.]. A Novel Bone Morphogenetic Protein 2 Mutant Mouse, nBmp2NLStm, Displays Impaired Intracellular Ca2+ Handling in Skeletal Muscle. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1003284
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1003284
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر