A Novel Bone Morphogenetic Protein 2 Mutant Mouse, nBmp2NLStm, Displays Impaired Intracellular Ca2+ Handling in Skeletal Muscle
Joint Authors
Bridgewater, Laura C.
Mayo, Jaime L.
Evanson, Bradley G.
Whitt, Megan E.
Dean, Spencer A.
Yates, Joshua D.
Holden, Devin N.
Schmidt, Alina D.
Fox, Christopher L.
Dhunghel, Saroj
Steed, Kevin S.
Adam, Michael M.
Nichols, Caitlin A.
Loganathan, Sampath K.
Barrow, Jeffery R.
Hancock, Chad R.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-28
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We recently reported a novel form of BMP2, designated nBMP2, which is translated from an alternative downstream start codon and is localized to the nucleus rather than secreted from the cell.
To examine the function of nBMP2 in the nucleus, we engineered a gene-targeted mutant mouse model (nBmp2NLStm) in which nBMP2 cannot be translocated to the nucleus.
Immunohistochemistry demonstrated the presence of nBMP2 staining in the myonuclei of wild type but not mutant skeletal muscle.
The nBmp2NLStm mouse exhibits altered function of skeletal muscle as demonstrated by a significant increase in the time required for relaxation following a stimulated twitch contraction.
Force frequency analysis showed elevated force production in mutant muscles compared to controls from 10 to 60 Hz stimulation frequency, consistent with the mutant muscle’s reduced ability to relax between rapidly stimulated contractions.
Muscle relaxation after contraction is mediated by the active transport of Ca2+ from the cytoplasm to the sarcoplasmic reticulum by sarco/endoplasmic reticulum Ca2+ ATPase (SERCA), and enzyme activity assays revealed that SERCA activity in skeletal muscle from nBmp2NLStm mice was reduced to approximately 80% of wild type.
These results suggest that nBMP2 plays a role in the establishment or maintenance of intracellular Ca2+ transport pathways in skeletal muscle.
American Psychological Association (APA)
Bridgewater, Laura C.& Mayo, Jaime L.& Evanson, Bradley G.& Whitt, Megan E.& Dean, Spencer A.& Yates, Joshua D.…[et al.]. 2013. A Novel Bone Morphogenetic Protein 2 Mutant Mouse, nBmp2NLStm, Displays Impaired Intracellular Ca2+ Handling in Skeletal Muscle. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1003284
Modern Language Association (MLA)
Bridgewater, Laura C.…[et al.]. A Novel Bone Morphogenetic Protein 2 Mutant Mouse, nBmp2NLStm, Displays Impaired Intracellular Ca2+ Handling in Skeletal Muscle. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1003284
American Medical Association (AMA)
Bridgewater, Laura C.& Mayo, Jaime L.& Evanson, Bradley G.& Whitt, Megan E.& Dean, Spencer A.& Yates, Joshua D.…[et al.]. A Novel Bone Morphogenetic Protein 2 Mutant Mouse, nBmp2NLStm, Displays Impaired Intracellular Ca2+ Handling in Skeletal Muscle. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1003284
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003284