In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells
المؤلفون المشاركون
Lim, Jung Jin
Kim, Hyung Joon
Kim, Kye-Seong
Hong, Jae Yup
Lee, Dong Ryul
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-12-24
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Unipotent spermatogonial stem cells (SSCs) can be transformed into ESC-like cells that exhibit pluripotency in vitro.
However, except for mouse models, their characterization and their origins have remained controversies in other models including humans.
This controversy has arisen primarily from the lack of the direct induction of ESC-like cells from well-characterized SSCs.
Thus, the aim of the present study was to find and characterize pluripotent human SSCs in in vitro cultures of characterized SSCs.
Human testicular tissues were dissociated and plated onto gelatin/laminin-coated dishes to isolate SSCs.
In the presence of growth factors SSCs formed multicellular clumps after 2–4 weeks of culture.
At passages 1 and 5, the clumps were dissociated and were then analyzed using markers of pluripotent cells.
The number of SSEA-4-positive cells was extremely low but increased gradually up to ~ 10% in the SSC clumps during culture.
Most of the SSEA-4-negative cells expressed markers for SSCs, and some cells coexpressed markers of both pluripotent and germ cells.
The pluripotent cells formed embryoid bodies and teratomas that contained derivatives of the three germ layers in SCID mice.
These results suggest that the pluripotent cells present within the clumps were derived directly from SSCs during in vitro culture.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lim, Jung Jin& Kim, Hyung Joon& Kim, Kye-Seong& Hong, Jae Yup& Lee, Dong Ryul. 2012. In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003383
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lim, Jung Jin…[et al.]. In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1003383
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lim, Jung Jin& Kim, Hyung Joon& Kim, Kye-Seong& Hong, Jae Yup& Lee, Dong Ryul. In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003383
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1003383
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر