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In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells
Joint Authors
Lim, Jung Jin
Kim, Hyung Joon
Kim, Kye-Seong
Hong, Jae Yup
Lee, Dong Ryul
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Unipotent spermatogonial stem cells (SSCs) can be transformed into ESC-like cells that exhibit pluripotency in vitro.
However, except for mouse models, their characterization and their origins have remained controversies in other models including humans.
This controversy has arisen primarily from the lack of the direct induction of ESC-like cells from well-characterized SSCs.
Thus, the aim of the present study was to find and characterize pluripotent human SSCs in in vitro cultures of characterized SSCs.
Human testicular tissues were dissociated and plated onto gelatin/laminin-coated dishes to isolate SSCs.
In the presence of growth factors SSCs formed multicellular clumps after 2–4 weeks of culture.
At passages 1 and 5, the clumps were dissociated and were then analyzed using markers of pluripotent cells.
The number of SSEA-4-positive cells was extremely low but increased gradually up to ~ 10% in the SSC clumps during culture.
Most of the SSEA-4-negative cells expressed markers for SSCs, and some cells coexpressed markers of both pluripotent and germ cells.
The pluripotent cells formed embryoid bodies and teratomas that contained derivatives of the three germ layers in SCID mice.
These results suggest that the pluripotent cells present within the clumps were derived directly from SSCs during in vitro culture.
American Psychological Association (APA)
Lim, Jung Jin& Kim, Hyung Joon& Kim, Kye-Seong& Hong, Jae Yup& Lee, Dong Ryul. 2012. In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003383
Modern Language Association (MLA)
Lim, Jung Jin…[et al.]. In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1003383
American Medical Association (AMA)
Lim, Jung Jin& Kim, Hyung Joon& Kim, Kye-Seong& Hong, Jae Yup& Lee, Dong Ryul. In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003383
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003383