Doxycycline Prevents Intimal Hyperplasia In Vitro and May Improve Patency of the Internal Thoracic Artery
المؤلفون المشاركون
Mannacio, Vito
Di Tommaso, Luigi
Antignano, Anita
Di Tommaso, Ettorino
Stassano, Paolo
Vosa, Carlo
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-08-22
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
|Objectives.
The development of intimal hyperplasia and graft failure is an important problem in cardiac surgery.
A fundamental process in intimal hyperplasia is the degradation of extracellular matrix by metalloproteases which induces the vascular smooth-muscle cells migration and sets the scene for graft atherosclerosis.
This study investigated whether doxycycline, a metalloproteases inhibitor, can prevent the intimal hyperplasia occurrence in cultured human internal mammary artery, thus extending graft patency.
Methods.
Segments of internal mammary artery from 20 consecutive patients were prepared and cultured for 2 weeks in serum-supplemented medium (control) or in medium supplemented with 10−5 M and 10−6 M doxycycline concentrations.
Tissues were fixed, sectioned, and stained, and neointimal thickness was measured by computer-aided image analysis.
Further sections were cultured and prepared for gel enzymography to measure the matrix metalloproteinase-2 and -9 levels.
Results.
At the end of the culture period, neointimal thickness was significantly (P=0.001) dose-dependently reduced in samples treated with doxycycline when compared with controls.
Gelatin enzymography demonstrated a reduction in values for both latent and active forms of metalloproteases.
Conclusions.
Doxycycline, in a model of internal mammary artery intimal hyperplasia, has a specific role in inhibiting metalloproteases activity and may prevent graft stenosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mannacio, Vito& Di Tommaso, Luigi& Antignano, Anita& Di Tommaso, Ettorino& Stassano, Paolo& Vosa, Carlo. 2013. Doxycycline Prevents Intimal Hyperplasia In Vitro and May Improve Patency of the Internal Thoracic Artery. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1003732
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mannacio, Vito…[et al.]. Doxycycline Prevents Intimal Hyperplasia In Vitro and May Improve Patency of the Internal Thoracic Artery. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1003732
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mannacio, Vito& Di Tommaso, Luigi& Antignano, Anita& Di Tommaso, Ettorino& Stassano, Paolo& Vosa, Carlo. Doxycycline Prevents Intimal Hyperplasia In Vitro and May Improve Patency of the Internal Thoracic Artery. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1003732
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1003732
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر