Doxycycline Prevents Intimal Hyperplasia In Vitro and May Improve Patency of the Internal Thoracic Artery
Joint Authors
Mannacio, Vito
Di Tommaso, Luigi
Antignano, Anita
Di Tommaso, Ettorino
Stassano, Paolo
Vosa, Carlo
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-22
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
|Objectives.
The development of intimal hyperplasia and graft failure is an important problem in cardiac surgery.
A fundamental process in intimal hyperplasia is the degradation of extracellular matrix by metalloproteases which induces the vascular smooth-muscle cells migration and sets the scene for graft atherosclerosis.
This study investigated whether doxycycline, a metalloproteases inhibitor, can prevent the intimal hyperplasia occurrence in cultured human internal mammary artery, thus extending graft patency.
Methods.
Segments of internal mammary artery from 20 consecutive patients were prepared and cultured for 2 weeks in serum-supplemented medium (control) or in medium supplemented with 10−5 M and 10−6 M doxycycline concentrations.
Tissues were fixed, sectioned, and stained, and neointimal thickness was measured by computer-aided image analysis.
Further sections were cultured and prepared for gel enzymography to measure the matrix metalloproteinase-2 and -9 levels.
Results.
At the end of the culture period, neointimal thickness was significantly (P=0.001) dose-dependently reduced in samples treated with doxycycline when compared with controls.
Gelatin enzymography demonstrated a reduction in values for both latent and active forms of metalloproteases.
Conclusions.
Doxycycline, in a model of internal mammary artery intimal hyperplasia, has a specific role in inhibiting metalloproteases activity and may prevent graft stenosis.
American Psychological Association (APA)
Mannacio, Vito& Di Tommaso, Luigi& Antignano, Anita& Di Tommaso, Ettorino& Stassano, Paolo& Vosa, Carlo. 2013. Doxycycline Prevents Intimal Hyperplasia In Vitro and May Improve Patency of the Internal Thoracic Artery. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1003732
Modern Language Association (MLA)
Mannacio, Vito…[et al.]. Doxycycline Prevents Intimal Hyperplasia In Vitro and May Improve Patency of the Internal Thoracic Artery. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1003732
American Medical Association (AMA)
Mannacio, Vito& Di Tommaso, Luigi& Antignano, Anita& Di Tommaso, Ettorino& Stassano, Paolo& Vosa, Carlo. Doxycycline Prevents Intimal Hyperplasia In Vitro and May Improve Patency of the Internal Thoracic Artery. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1003732
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003732