The Role of Bone Marrow Mesenchymal Stem Cells in the Treatment of Acute Liver Failure

المؤلفون المشاركون

Yuan, Shufang
Jiang, Tao
Sun, Lihua
Zheng, Rongjiong
Ahat, Nizam
Zhang, Yuexin

المصدر

BioMed Research International

العدد

المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2013-11-10

دولة النشر

مصر

عدد الصفحات

9

التخصصات الرئيسية

الطب البشري

الملخص EN

This study is to investigate the effects of bone marrow mesenchymal stem cell (BMSC) transplantation on acute liver failure (ALF).

Methods.

BMSCs were separated from rat bone marrow, cultured, and identified by flow cytometry.

Rat model with ALF was established by injecting D-galactosamine and lipopolysaccharide.

Rats were randomly divided into the control group and BMSC transplantation group.

The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured at 24 h, 120 h, and 168 h after BMSC transplantation.

Apoptosis was detected by TUNEL assay.

The expression of VEGF and AFP proteins was detected by immunofluorescence.

Caspase-1 and IL-18 proteins and mRNA were detected by immunohistochemistry and RT-PCR.

Results.

Compared with the control group, levels of ALT, AST, caspase-1 and IL-18 proteins, and mRNA in the transplantation group were significantly lower at 120 h and 168 h after BMSCs transplantation.

Apoptosis was inhibited by BMSCs transplantation.

The VEGF protein levels were increased with the improvement of liver function, and the AFP protein levels were increased with the deterioration of the liver function after BMSCs transplantation.

Conclusions.

BMSCs transplantation can improve liver function and inhibit hepatocyte apoptosis as well as promote hepatocyte proliferation in rat model with ALF.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Yuan, Shufang& Jiang, Tao& Sun, Lihua& Zheng, Rongjiong& Ahat, Nizam& Zhang, Yuexin. 2013. The Role of Bone Marrow Mesenchymal Stem Cells in the Treatment of Acute Liver Failure. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003800

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Yuan, Shufang…[et al.]. The Role of Bone Marrow Mesenchymal Stem Cells in the Treatment of Acute Liver Failure. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1003800

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Yuan, Shufang& Jiang, Tao& Sun, Lihua& Zheng, Rongjiong& Ahat, Nizam& Zhang, Yuexin. The Role of Bone Marrow Mesenchymal Stem Cells in the Treatment of Acute Liver Failure. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003800

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1003800