The Role of Bone Marrow Mesenchymal Stem Cells in the Treatment of Acute Liver Failure

Joint Authors

Yuan, Shufang
Jiang, Tao
Sun, Lihua
Zheng, Rongjiong
Ahat, Nizam
Zhang, Yuexin

Source

BioMed Research International

Issue

Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2013-11-10

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Medicine

Abstract EN

This study is to investigate the effects of bone marrow mesenchymal stem cell (BMSC) transplantation on acute liver failure (ALF).

Methods.

BMSCs were separated from rat bone marrow, cultured, and identified by flow cytometry.

Rat model with ALF was established by injecting D-galactosamine and lipopolysaccharide.

Rats were randomly divided into the control group and BMSC transplantation group.

The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured at 24 h, 120 h, and 168 h after BMSC transplantation.

Apoptosis was detected by TUNEL assay.

The expression of VEGF and AFP proteins was detected by immunofluorescence.

Caspase-1 and IL-18 proteins and mRNA were detected by immunohistochemistry and RT-PCR.

Results.

Compared with the control group, levels of ALT, AST, caspase-1 and IL-18 proteins, and mRNA in the transplantation group were significantly lower at 120 h and 168 h after BMSCs transplantation.

Apoptosis was inhibited by BMSCs transplantation.

The VEGF protein levels were increased with the improvement of liver function, and the AFP protein levels were increased with the deterioration of the liver function after BMSCs transplantation.

Conclusions.

BMSCs transplantation can improve liver function and inhibit hepatocyte apoptosis as well as promote hepatocyte proliferation in rat model with ALF.

American Psychological Association (APA)

Yuan, Shufang& Jiang, Tao& Sun, Lihua& Zheng, Rongjiong& Ahat, Nizam& Zhang, Yuexin. 2013. The Role of Bone Marrow Mesenchymal Stem Cells in the Treatment of Acute Liver Failure. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003800

Modern Language Association (MLA)

Yuan, Shufang…[et al.]. The Role of Bone Marrow Mesenchymal Stem Cells in the Treatment of Acute Liver Failure. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1003800

American Medical Association (AMA)

Yuan, Shufang& Jiang, Tao& Sun, Lihua& Zheng, Rongjiong& Ahat, Nizam& Zhang, Yuexin. The Role of Bone Marrow Mesenchymal Stem Cells in the Treatment of Acute Liver Failure. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003800

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1003800