The Role of Bone Marrow Mesenchymal Stem Cells in the Treatment of Acute Liver Failure
Joint Authors
Yuan, Shufang
Jiang, Tao
Sun, Lihua
Zheng, Rongjiong
Ahat, Nizam
Zhang, Yuexin
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This study is to investigate the effects of bone marrow mesenchymal stem cell (BMSC) transplantation on acute liver failure (ALF).
Methods.
BMSCs were separated from rat bone marrow, cultured, and identified by flow cytometry.
Rat model with ALF was established by injecting D-galactosamine and lipopolysaccharide.
Rats were randomly divided into the control group and BMSC transplantation group.
The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured at 24 h, 120 h, and 168 h after BMSC transplantation.
Apoptosis was detected by TUNEL assay.
The expression of VEGF and AFP proteins was detected by immunofluorescence.
Caspase-1 and IL-18 proteins and mRNA were detected by immunohistochemistry and RT-PCR.
Results.
Compared with the control group, levels of ALT, AST, caspase-1 and IL-18 proteins, and mRNA in the transplantation group were significantly lower at 120 h and 168 h after BMSCs transplantation.
Apoptosis was inhibited by BMSCs transplantation.
The VEGF protein levels were increased with the improvement of liver function, and the AFP protein levels were increased with the deterioration of the liver function after BMSCs transplantation.
Conclusions.
BMSCs transplantation can improve liver function and inhibit hepatocyte apoptosis as well as promote hepatocyte proliferation in rat model with ALF.
American Psychological Association (APA)
Yuan, Shufang& Jiang, Tao& Sun, Lihua& Zheng, Rongjiong& Ahat, Nizam& Zhang, Yuexin. 2013. The Role of Bone Marrow Mesenchymal Stem Cells in the Treatment of Acute Liver Failure. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003800
Modern Language Association (MLA)
Yuan, Shufang…[et al.]. The Role of Bone Marrow Mesenchymal Stem Cells in the Treatment of Acute Liver Failure. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1003800
American Medical Association (AMA)
Yuan, Shufang& Jiang, Tao& Sun, Lihua& Zheng, Rongjiong& Ahat, Nizam& Zhang, Yuexin. The Role of Bone Marrow Mesenchymal Stem Cells in the Treatment of Acute Liver Failure. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003800
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003800