Transfected Early Growth Response Gene-1 DNA Enzyme Prevents Stenosis and Occlusion of Autogenous Vein Graft In Vivo
المؤلفون المشاركون
Zhuang, Qi
Liu, Chengwei
Zhang, Xuesong
Wang, Shi
Cheng, Mingxun
Liu, Chuanyu
Jiang, Xiao-Hong
Tang, Ying
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-03-13
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The aim of this study was to detect the inhibitory action of the early growth response gene-1 DNA enzyme (EDRz) as a carrying agent by liposomes on vascular smooth muscle cell proliferation and intimal hyperplasia.
An autogenous vein graft model was established.
EDRz was transfected to the graft vein.
The vein graft samples were obtained on each time point after surgery.
The expression of the EDRz transfected in the vein graft was detected using a fluorescent microscope.
Early growth response gene-1 (Egr-1) mRNA was measured using reverse transcription-PCR and in situ hybridization.
And the protein expression of Egr-1 was detected by using western blot and immunohistochemistry analyses.
EDRz was located at the media of the vein graft from 2 to 24 h, 7 h after grafting.
The Egr-1 protein was mainly located in the medial VSMCs, monocytes, and endothelium cells during the early phase of the vein graft.
The degree of VSMC proliferation and thickness of intima were obviously relieved compared with the no-gene therapy group.
EDRz can reduce Egr-1 expression in autogenous vein grafts, effectively restrain VSMC proliferation and intimal hyperplasia, and prevent vascular stenosis and occlusion after vein graft.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Chengwei& Zhang, Xuesong& Wang, Shi& Cheng, Mingxun& Liu, Chuanyu& Tang, Ying…[et al.]. 2013. Transfected Early Growth Response Gene-1 DNA Enzyme Prevents Stenosis and Occlusion of Autogenous Vein Graft In Vivo. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1003979
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Chengwei…[et al.]. Transfected Early Growth Response Gene-1 DNA Enzyme Prevents Stenosis and Occlusion of Autogenous Vein Graft In Vivo. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1003979
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Chengwei& Zhang, Xuesong& Wang, Shi& Cheng, Mingxun& Liu, Chuanyu& Tang, Ying…[et al.]. Transfected Early Growth Response Gene-1 DNA Enzyme Prevents Stenosis and Occlusion of Autogenous Vein Graft In Vivo. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1003979
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1003979
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر