Transfected Early Growth Response Gene-1 DNA Enzyme Prevents Stenosis and Occlusion of Autogenous Vein Graft In Vivo
Joint Authors
Zhuang, Qi
Liu, Chengwei
Zhang, Xuesong
Wang, Shi
Cheng, Mingxun
Liu, Chuanyu
Jiang, Xiao-Hong
Tang, Ying
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The aim of this study was to detect the inhibitory action of the early growth response gene-1 DNA enzyme (EDRz) as a carrying agent by liposomes on vascular smooth muscle cell proliferation and intimal hyperplasia.
An autogenous vein graft model was established.
EDRz was transfected to the graft vein.
The vein graft samples were obtained on each time point after surgery.
The expression of the EDRz transfected in the vein graft was detected using a fluorescent microscope.
Early growth response gene-1 (Egr-1) mRNA was measured using reverse transcription-PCR and in situ hybridization.
And the protein expression of Egr-1 was detected by using western blot and immunohistochemistry analyses.
EDRz was located at the media of the vein graft from 2 to 24 h, 7 h after grafting.
The Egr-1 protein was mainly located in the medial VSMCs, monocytes, and endothelium cells during the early phase of the vein graft.
The degree of VSMC proliferation and thickness of intima were obviously relieved compared with the no-gene therapy group.
EDRz can reduce Egr-1 expression in autogenous vein grafts, effectively restrain VSMC proliferation and intimal hyperplasia, and prevent vascular stenosis and occlusion after vein graft.
American Psychological Association (APA)
Liu, Chengwei& Zhang, Xuesong& Wang, Shi& Cheng, Mingxun& Liu, Chuanyu& Tang, Ying…[et al.]. 2013. Transfected Early Growth Response Gene-1 DNA Enzyme Prevents Stenosis and Occlusion of Autogenous Vein Graft In Vivo. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1003979
Modern Language Association (MLA)
Liu, Chengwei…[et al.]. Transfected Early Growth Response Gene-1 DNA Enzyme Prevents Stenosis and Occlusion of Autogenous Vein Graft In Vivo. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1003979
American Medical Association (AMA)
Liu, Chengwei& Zhang, Xuesong& Wang, Shi& Cheng, Mingxun& Liu, Chuanyu& Tang, Ying…[et al.]. Transfected Early Growth Response Gene-1 DNA Enzyme Prevents Stenosis and Occlusion of Autogenous Vein Graft In Vivo. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1003979
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003979