Ascorbic Acid and BSA Protein in Solution and Films: Interaction and Surface Morphological Structure
المؤلفون المشاركون
Silva, Josmary R.
Trescher, Tarquin F.
Maciel, Rafael R. G.
de Almeida, Adriele A.
Godinho, Odin G. C.
Gorza, Filipe D. S.
Pedro, Graciela C.
de Souza, Nara C.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-07-25
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
This paper reports on the study of the interactions between ascorbic acid (AA) and bovine serum albumin (BSA) in aqueous solution as well as in films (BSA/AA films) prepared by the layer-by-layer technique.
Regarding to solution studies, a hyperchromism (in the range of ultraviolet) was found as a function of AA concentration, which suggested the formation of aggregates from AA and BSA.
Binding constant, K, determined for aggregates from BSA and AA was found to be about 102 M−1, which indicated low affinity of AA with BSA.
For the BSA/AA films, it was also noted that the AA adsorption process and surface morphological structures depended on AA concentration.
By changing the contact time between the AA and BSA, a hypochromism was revealed, which was associated to decrease of accessibility of solvent to tryptophan due to formation of aggregates.
Furthermore, different morphological structures of aggregates were observed, which were attributed to the diffusion-limited aggregation.
Since most of studies of interactions of drugs and proteins are performed in solution, the analysis of these processes by using films can be very valuable because this kind of system is able to employ several techniques of investigation in solid state.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Maciel, Rafael R. G.& de Almeida, Adriele A.& Godinho, Odin G. C.& Gorza, Filipe D. S.& Pedro, Graciela C.& Trescher, Tarquin F.…[et al.]. 2013. Ascorbic Acid and BSA Protein in Solution and Films: Interaction and Surface Morphological Structure. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004347
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Maciel, Rafael R. G.…[et al.]. Ascorbic Acid and BSA Protein in Solution and Films: Interaction and Surface Morphological Structure. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1004347
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Maciel, Rafael R. G.& de Almeida, Adriele A.& Godinho, Odin G. C.& Gorza, Filipe D. S.& Pedro, Graciela C.& Trescher, Tarquin F.…[et al.]. Ascorbic Acid and BSA Protein in Solution and Films: Interaction and Surface Morphological Structure. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004347
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1004347
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر