Ascorbic Acid and BSA Protein in Solution and Films: Interaction and Surface Morphological Structure
Joint Authors
Silva, Josmary R.
Trescher, Tarquin F.
Maciel, Rafael R. G.
de Almeida, Adriele A.
Godinho, Odin G. C.
Gorza, Filipe D. S.
Pedro, Graciela C.
de Souza, Nara C.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-25
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This paper reports on the study of the interactions between ascorbic acid (AA) and bovine serum albumin (BSA) in aqueous solution as well as in films (BSA/AA films) prepared by the layer-by-layer technique.
Regarding to solution studies, a hyperchromism (in the range of ultraviolet) was found as a function of AA concentration, which suggested the formation of aggregates from AA and BSA.
Binding constant, K, determined for aggregates from BSA and AA was found to be about 102 M−1, which indicated low affinity of AA with BSA.
For the BSA/AA films, it was also noted that the AA adsorption process and surface morphological structures depended on AA concentration.
By changing the contact time between the AA and BSA, a hypochromism was revealed, which was associated to decrease of accessibility of solvent to tryptophan due to formation of aggregates.
Furthermore, different morphological structures of aggregates were observed, which were attributed to the diffusion-limited aggregation.
Since most of studies of interactions of drugs and proteins are performed in solution, the analysis of these processes by using films can be very valuable because this kind of system is able to employ several techniques of investigation in solid state.
American Psychological Association (APA)
Maciel, Rafael R. G.& de Almeida, Adriele A.& Godinho, Odin G. C.& Gorza, Filipe D. S.& Pedro, Graciela C.& Trescher, Tarquin F.…[et al.]. 2013. Ascorbic Acid and BSA Protein in Solution and Films: Interaction and Surface Morphological Structure. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004347
Modern Language Association (MLA)
Maciel, Rafael R. G.…[et al.]. Ascorbic Acid and BSA Protein in Solution and Films: Interaction and Surface Morphological Structure. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1004347
American Medical Association (AMA)
Maciel, Rafael R. G.& de Almeida, Adriele A.& Godinho, Odin G. C.& Gorza, Filipe D. S.& Pedro, Graciela C.& Trescher, Tarquin F.…[et al.]. Ascorbic Acid and BSA Protein in Solution and Films: Interaction and Surface Morphological Structure. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004347
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004347