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Development of an Immunochromatographic Test Strip for Detection of Cholera Toxin
المؤلفون المشاركون
Yamasaki, Eiki
Sakamoto, Ryuta
Matsumoto, Takashi
Morimatsu, Fumiki
Kurazono, Takayuki
Hiroi, Toyoko
Nair, G. Balakrish
Kurazono, Hisao
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-11-07
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Because cholera toxin (CT) is responsible for most of the symptoms induced by Vibrio cholerae infection, detection of CT is critical for diagnosis of the disease.
In this study, we constructed an immunochromatographic test strip for detection of CT (CT-IC) with polyclonal antibodies developed against purified recombinant whole CT protein.
The detection limit of the CT-IC was 10 ng/mL of purified recombinant CT, and it could detect the CT in culture supernatant of all 15 toxigenic V.
cholerae isolates examined, whereas no false-positive signal was detected in all 5 nontoxigenic V.
cholerae isolates examined.
The specificity of the CT-IC was examined with recombinant heat-labile toxin (LT), which shares high homology with CT, and it was revealed that the minimum detection limit for LT was 100 times higher than that for CT.
In addition, lt gene-positive enterotoxigenic Escherichia coli (ETEC) was examined by CT-IC.
The false-positive signals were observed in 3 out of 12 ETEC isolates, but these signals were considerably faint.
The CT-IC did not develop false-positive signals with all 7 V.
parahaemolyticus isolates.
These results showed the high specificity of CT-IC and the feasible use of it for the detection and surveillance of toxigenic V.
cholerae.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yamasaki, Eiki& Sakamoto, Ryuta& Matsumoto, Takashi& Morimatsu, Fumiki& Kurazono, Takayuki& Hiroi, Toyoko…[et al.]. 2013. Development of an Immunochromatographic Test Strip for Detection of Cholera Toxin. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004836
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yamasaki, Eiki…[et al.]. Development of an Immunochromatographic Test Strip for Detection of Cholera Toxin. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1004836
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yamasaki, Eiki& Sakamoto, Ryuta& Matsumoto, Takashi& Morimatsu, Fumiki& Kurazono, Takayuki& Hiroi, Toyoko…[et al.]. Development of an Immunochromatographic Test Strip for Detection of Cholera Toxin. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004836
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1004836
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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