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Development of an Immunochromatographic Test Strip for Detection of Cholera Toxin
Joint Authors
Yamasaki, Eiki
Sakamoto, Ryuta
Matsumoto, Takashi
Morimatsu, Fumiki
Kurazono, Takayuki
Hiroi, Toyoko
Nair, G. Balakrish
Kurazono, Hisao
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Because cholera toxin (CT) is responsible for most of the symptoms induced by Vibrio cholerae infection, detection of CT is critical for diagnosis of the disease.
In this study, we constructed an immunochromatographic test strip for detection of CT (CT-IC) with polyclonal antibodies developed against purified recombinant whole CT protein.
The detection limit of the CT-IC was 10 ng/mL of purified recombinant CT, and it could detect the CT in culture supernatant of all 15 toxigenic V.
cholerae isolates examined, whereas no false-positive signal was detected in all 5 nontoxigenic V.
cholerae isolates examined.
The specificity of the CT-IC was examined with recombinant heat-labile toxin (LT), which shares high homology with CT, and it was revealed that the minimum detection limit for LT was 100 times higher than that for CT.
In addition, lt gene-positive enterotoxigenic Escherichia coli (ETEC) was examined by CT-IC.
The false-positive signals were observed in 3 out of 12 ETEC isolates, but these signals were considerably faint.
The CT-IC did not develop false-positive signals with all 7 V.
parahaemolyticus isolates.
These results showed the high specificity of CT-IC and the feasible use of it for the detection and surveillance of toxigenic V.
cholerae.
American Psychological Association (APA)
Yamasaki, Eiki& Sakamoto, Ryuta& Matsumoto, Takashi& Morimatsu, Fumiki& Kurazono, Takayuki& Hiroi, Toyoko…[et al.]. 2013. Development of an Immunochromatographic Test Strip for Detection of Cholera Toxin. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004836
Modern Language Association (MLA)
Yamasaki, Eiki…[et al.]. Development of an Immunochromatographic Test Strip for Detection of Cholera Toxin. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1004836
American Medical Association (AMA)
Yamasaki, Eiki& Sakamoto, Ryuta& Matsumoto, Takashi& Morimatsu, Fumiki& Kurazono, Takayuki& Hiroi, Toyoko…[et al.]. Development of an Immunochromatographic Test Strip for Detection of Cholera Toxin. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004836
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004836