Hyaluronic Acid Derived from Other Streptococci Supports Streptococcus pneumoniae In Vitro Biofilm Formation
المؤلفون المشاركون
Yadav, Mukesh Kumar
Chae, Sung-Won
Park, Kyeongsoon
Song, Jae-Jun
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-19
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
We investigate the role of hyaluronic acid (HA) on S.
pneumoniae in vitro biofilm formation and evaluate gene expressions of virulence and/or biofilm related genes.
Biofilms were grown in medium supplied with HA derived from capsule of Streptococcus equi.
The biomasses of biofilms were detected by crystal-violet (CV) microtiter plate assay, and the morphology was viewed under scanning electron microscope (SEM).
The gene expressions were assessed by relative quantitative RT-PCR.
The results showed that the HA support pneumococcal growth in planktonic form and within biofilms.
The CV-microtiter plate assay detected significantly increased biofilm growth in medium containing HA.
The SEM analysis revealed thick and organized biofilms in positive control and HA supplemented medium.
The nanA, nanB, bgaA, strH, luxS, hysA, ugl, and PST-EIIA encoding genes were significantly upregulated in the planktonic cells grown in presence of HA, while the lytA and comA genes were downregulated.
Similarly the luxS, hysA, ugl, and PST-EIIA encoding genes were significantly upregulated by more than 2-folds in HA biofilms.
The results of this study indicate that the HA derived from capsule of S.
equi supports pneumococcal growth in planktonic state and within biofilms and upregulated virulence and biofilm related genes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yadav, Mukesh Kumar& Chae, Sung-Won& Park, Kyeongsoon& Song, Jae-Jun. 2013. Hyaluronic Acid Derived from Other Streptococci Supports Streptococcus pneumoniae In Vitro Biofilm Formation. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004874
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yadav, Mukesh Kumar…[et al.]. Hyaluronic Acid Derived from Other Streptococci Supports Streptococcus pneumoniae In Vitro Biofilm Formation. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1004874
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yadav, Mukesh Kumar& Chae, Sung-Won& Park, Kyeongsoon& Song, Jae-Jun. Hyaluronic Acid Derived from Other Streptococci Supports Streptococcus pneumoniae In Vitro Biofilm Formation. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004874
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1004874
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر