Hyaluronic Acid Derived from Other Streptococci Supports Streptococcus pneumoniae In Vitro Biofilm Formation
Joint Authors
Yadav, Mukesh Kumar
Chae, Sung-Won
Park, Kyeongsoon
Song, Jae-Jun
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We investigate the role of hyaluronic acid (HA) on S.
pneumoniae in vitro biofilm formation and evaluate gene expressions of virulence and/or biofilm related genes.
Biofilms were grown in medium supplied with HA derived from capsule of Streptococcus equi.
The biomasses of biofilms were detected by crystal-violet (CV) microtiter plate assay, and the morphology was viewed under scanning electron microscope (SEM).
The gene expressions were assessed by relative quantitative RT-PCR.
The results showed that the HA support pneumococcal growth in planktonic form and within biofilms.
The CV-microtiter plate assay detected significantly increased biofilm growth in medium containing HA.
The SEM analysis revealed thick and organized biofilms in positive control and HA supplemented medium.
The nanA, nanB, bgaA, strH, luxS, hysA, ugl, and PST-EIIA encoding genes were significantly upregulated in the planktonic cells grown in presence of HA, while the lytA and comA genes were downregulated.
Similarly the luxS, hysA, ugl, and PST-EIIA encoding genes were significantly upregulated by more than 2-folds in HA biofilms.
The results of this study indicate that the HA derived from capsule of S.
equi supports pneumococcal growth in planktonic state and within biofilms and upregulated virulence and biofilm related genes.
American Psychological Association (APA)
Yadav, Mukesh Kumar& Chae, Sung-Won& Park, Kyeongsoon& Song, Jae-Jun. 2013. Hyaluronic Acid Derived from Other Streptococci Supports Streptococcus pneumoniae In Vitro Biofilm Formation. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004874
Modern Language Association (MLA)
Yadav, Mukesh Kumar…[et al.]. Hyaluronic Acid Derived from Other Streptococci Supports Streptococcus pneumoniae In Vitro Biofilm Formation. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1004874
American Medical Association (AMA)
Yadav, Mukesh Kumar& Chae, Sung-Won& Park, Kyeongsoon& Song, Jae-Jun. Hyaluronic Acid Derived from Other Streptococci Supports Streptococcus pneumoniae In Vitro Biofilm Formation. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004874
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004874