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Formulation Development and Evaluation of Hybrid Nanocarrier for Cancer Therapy: Taguchi Orthogonal Array Based Design
المؤلفون المشاركون
Tekade, Rakesh K.
Chougule, Mahavir B.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-18، 18ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-11
دولة النشر
مصر
عدد الصفحات
18
التخصصات الرئيسية
الملخص EN
Taguchi orthogonal array design is a statistical approach that helps to overcome limitations associated with time consuming full factorial experimental design.
In this study, the Taguchi orthogonal array design was applied to establish the optimum conditions for bovine serum albumin (BSA) nanocarrier (ANC) preparation.
Taguchi method with L9 type of robust orthogonal array design was adopted to optimize the experimental conditions.
Three key dependent factors namely, BSA concentration (% w/v), volume of BSA solution to total ethanol ratio (v : v), and concentration of diluted ethanolic aqueous solution (% v/v), were studied at three levels 3%, 4%, and 5% w/v; 1 : 0.75, 1 : 0.90, and 1 : 1.05 v/v; 40%, 70%, and 100% v/v, respectively.
The ethanolic aqueous solution was used to impart less harsh condition for desolvation and attain controlled nanoparticle formation.
The interaction plot studies inferred the ethanolic aqueous solution concentration to be the most influential parameter that affects the particle size of nanoformulation.
This method (BSA, 4% w/v; volume of BSA solution to total ethanol ratio, 1 : 0.90 v/v; concentration of diluted ethanolic solution, 70% v/v) was able to successfully develop Gemcitabine (G) loaded modified albumin nanocarrier (M-ANC-G) of size 25.07±2.81 nm (ζ=-23.03±1.015 mV) as against to 78.01±4.99 nm (ζ=-24.88±1.37 mV) using conventional method albumin nanocarrier (C-ANC-G).
Hybrid nanocarriers were generated by chitosan layering (solvent gelation technique) of respective ANC to form C-HNC-G and M-HNC-G of sizes 125.29±5.62 nm (ζ=12.01±0.51 mV) and 46.28±2.21 nm (ζ=15.05±0.39 mV), respectively.
Zeta potential, entrapment, in vitro release, and pH-based stability studies were investigated and influence of formulation parameters are discussed.
Cell-line-based cytotoxicity assay (A549 and H460 cells) and cell internalization assay (H460 cell line) were performed to assess the influence on the bioperformance of these nanoformulations.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tekade, Rakesh K.& Chougule, Mahavir B.. 2013. Formulation Development and Evaluation of Hybrid Nanocarrier for Cancer Therapy: Taguchi Orthogonal Array Based Design. BioMed Research International،Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-1004932
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tekade, Rakesh K.& Chougule, Mahavir B.. Formulation Development and Evaluation of Hybrid Nanocarrier for Cancer Therapy: Taguchi Orthogonal Array Based Design. BioMed Research International No. 2013 (2013), pp.1-18.
https://search.emarefa.net/detail/BIM-1004932
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tekade, Rakesh K.& Chougule, Mahavir B.. Formulation Development and Evaluation of Hybrid Nanocarrier for Cancer Therapy: Taguchi Orthogonal Array Based Design. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-1004932
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1004932
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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