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Formulation Development and Evaluation of Hybrid Nanocarrier for Cancer Therapy: Taguchi Orthogonal Array Based Design
Joint Authors
Tekade, Rakesh K.
Chougule, Mahavir B.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-11
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Taguchi orthogonal array design is a statistical approach that helps to overcome limitations associated with time consuming full factorial experimental design.
In this study, the Taguchi orthogonal array design was applied to establish the optimum conditions for bovine serum albumin (BSA) nanocarrier (ANC) preparation.
Taguchi method with L9 type of robust orthogonal array design was adopted to optimize the experimental conditions.
Three key dependent factors namely, BSA concentration (% w/v), volume of BSA solution to total ethanol ratio (v : v), and concentration of diluted ethanolic aqueous solution (% v/v), were studied at three levels 3%, 4%, and 5% w/v; 1 : 0.75, 1 : 0.90, and 1 : 1.05 v/v; 40%, 70%, and 100% v/v, respectively.
The ethanolic aqueous solution was used to impart less harsh condition for desolvation and attain controlled nanoparticle formation.
The interaction plot studies inferred the ethanolic aqueous solution concentration to be the most influential parameter that affects the particle size of nanoformulation.
This method (BSA, 4% w/v; volume of BSA solution to total ethanol ratio, 1 : 0.90 v/v; concentration of diluted ethanolic solution, 70% v/v) was able to successfully develop Gemcitabine (G) loaded modified albumin nanocarrier (M-ANC-G) of size 25.07±2.81 nm (ζ=-23.03±1.015 mV) as against to 78.01±4.99 nm (ζ=-24.88±1.37 mV) using conventional method albumin nanocarrier (C-ANC-G).
Hybrid nanocarriers were generated by chitosan layering (solvent gelation technique) of respective ANC to form C-HNC-G and M-HNC-G of sizes 125.29±5.62 nm (ζ=12.01±0.51 mV) and 46.28±2.21 nm (ζ=15.05±0.39 mV), respectively.
Zeta potential, entrapment, in vitro release, and pH-based stability studies were investigated and influence of formulation parameters are discussed.
Cell-line-based cytotoxicity assay (A549 and H460 cells) and cell internalization assay (H460 cell line) were performed to assess the influence on the bioperformance of these nanoformulations.
American Psychological Association (APA)
Tekade, Rakesh K.& Chougule, Mahavir B.. 2013. Formulation Development and Evaluation of Hybrid Nanocarrier for Cancer Therapy: Taguchi Orthogonal Array Based Design. BioMed Research International،Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-1004932
Modern Language Association (MLA)
Tekade, Rakesh K.& Chougule, Mahavir B.. Formulation Development and Evaluation of Hybrid Nanocarrier for Cancer Therapy: Taguchi Orthogonal Array Based Design. BioMed Research International No. 2013 (2013), pp.1-18.
https://search.emarefa.net/detail/BIM-1004932
American Medical Association (AMA)
Tekade, Rakesh K.& Chougule, Mahavir B.. Formulation Development and Evaluation of Hybrid Nanocarrier for Cancer Therapy: Taguchi Orthogonal Array Based Design. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-1004932
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004932