MHC Universal Cells Survive in an Allogeneic Environment after Incompatible Transplantation

المؤلفون المشاركون

Figueiredo, Constança
Wedekind, Dirk
Müller, Thomas
Vahlsing, Stefanie
Horn, Peter A.
Seltsam, Axel
Blasczyk, Rainer

المصدر

BioMed Research International

العدد

المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2013-10-09

دولة النشر

مصر

عدد الصفحات

12

التخصصات الرئيسية

الطب البشري

الملخص EN

Cell, tissue, and organ transplants are commonly performed for the treatment of different diseases.

However, major histocompatibility complex (MHC) diversity often prevents complete donor-recipient matching, resulting in graft rejection.

This study evaluates in a preclinical model the capacity of MHC class I-silenced cells to engraft and grow upon allogeneic transplantation.

Short hairpin RNA targeting β2-microglobulin (RN_shβ2m) was delivered into fibroblasts derived from LEW/Ztm (RT1l) (RT1-Al) rats using a lentiviral-based vector.

MHC class I (RT1-A-) expressing and -silenced cells were injected subcutaneously in LEW rats (RT1l) and MHC-congenic LEW.1W rats (RT1u), respectively.

Cell engraftment and the status of the immune response were monitored for eight weeks after transplantation.

In contrast to RT1-A-expressing cells, RT1-A-silenced fibroblasts became engrafted and were still detectable eight weeks after allogeneic transplantation.

Plasma levels of proinflammatory cytokines IL-1α, IL-1β, IL-6, TNF-α, and IFN-γ were significantly higher in animals transplanted with RT1-A-expressing cells than in those receiving RT1-A-silenced cells.

Furthermore, alloantigen-specific T-cell proliferation rates derived from rats receiving RT1-A-expressing cells were higher than those in rats transplanted with RT1-A-silenced cells.

These data suggest that silencing MHC class I expression might overcome the histocompatibility barrier, potentially opening up new avenues in the field of cell transplantation and regenerative medicine.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Figueiredo, Constança& Wedekind, Dirk& Müller, Thomas& Vahlsing, Stefanie& Horn, Peter A.& Seltsam, Axel…[et al.]. 2013. MHC Universal Cells Survive in an Allogeneic Environment after Incompatible Transplantation. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1005138

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Figueiredo, Constança…[et al.]. MHC Universal Cells Survive in an Allogeneic Environment after Incompatible Transplantation. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1005138

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Figueiredo, Constança& Wedekind, Dirk& Müller, Thomas& Vahlsing, Stefanie& Horn, Peter A.& Seltsam, Axel…[et al.]. MHC Universal Cells Survive in an Allogeneic Environment after Incompatible Transplantation. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1005138

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1005138