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MHC Universal Cells Survive in an Allogeneic Environment after Incompatible Transplantation
Joint Authors
Figueiredo, Constança
Wedekind, Dirk
Müller, Thomas
Vahlsing, Stefanie
Horn, Peter A.
Seltsam, Axel
Blasczyk, Rainer
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-09
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Cell, tissue, and organ transplants are commonly performed for the treatment of different diseases.
However, major histocompatibility complex (MHC) diversity often prevents complete donor-recipient matching, resulting in graft rejection.
This study evaluates in a preclinical model the capacity of MHC class I-silenced cells to engraft and grow upon allogeneic transplantation.
Short hairpin RNA targeting β2-microglobulin (RN_shβ2m) was delivered into fibroblasts derived from LEW/Ztm (RT1l) (RT1-Al) rats using a lentiviral-based vector.
MHC class I (RT1-A-) expressing and -silenced cells were injected subcutaneously in LEW rats (RT1l) and MHC-congenic LEW.1W rats (RT1u), respectively.
Cell engraftment and the status of the immune response were monitored for eight weeks after transplantation.
In contrast to RT1-A-expressing cells, RT1-A-silenced fibroblasts became engrafted and were still detectable eight weeks after allogeneic transplantation.
Plasma levels of proinflammatory cytokines IL-1α, IL-1β, IL-6, TNF-α, and IFN-γ were significantly higher in animals transplanted with RT1-A-expressing cells than in those receiving RT1-A-silenced cells.
Furthermore, alloantigen-specific T-cell proliferation rates derived from rats receiving RT1-A-expressing cells were higher than those in rats transplanted with RT1-A-silenced cells.
These data suggest that silencing MHC class I expression might overcome the histocompatibility barrier, potentially opening up new avenues in the field of cell transplantation and regenerative medicine.
American Psychological Association (APA)
Figueiredo, Constança& Wedekind, Dirk& Müller, Thomas& Vahlsing, Stefanie& Horn, Peter A.& Seltsam, Axel…[et al.]. 2013. MHC Universal Cells Survive in an Allogeneic Environment after Incompatible Transplantation. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1005138
Modern Language Association (MLA)
Figueiredo, Constança…[et al.]. MHC Universal Cells Survive in an Allogeneic Environment after Incompatible Transplantation. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1005138
American Medical Association (AMA)
Figueiredo, Constança& Wedekind, Dirk& Müller, Thomas& Vahlsing, Stefanie& Horn, Peter A.& Seltsam, Axel…[et al.]. MHC Universal Cells Survive in an Allogeneic Environment after Incompatible Transplantation. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1005138
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005138