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Molecular Prevalence of Babesia bigemina and Trypanosoma evansi in Dairy Animals from Punjab, India, by Duplex PCR: A Step Forward to the Detection and Management of Concurrent Latent Infections
المؤلفون المشاركون
Sharma, Amrita
Singla, Lachhman Das
Tuli, Ashuma
Kaur, Paramjit
Batth, Balwinder Kaur
Javed, Mohammed
Juyal, Prayag Dutt
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-08-29
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Specific duplex polymerase chain reaction (PCR) was employed on 411 (386 cattle and 25 buffaloes) blood samples of dairy animals from 9 districts of Punjab, India, for simultaneous detection of Babesia bigemina and Trypanosoma evansi.
The results were compared and correlated with conventional Giemsa stained thin blood smear (GSTBS) examination and haematological alterations to know the clinical status and pathogenicity of infections.
The Bg3/Bg4 and TR3/TR4 primers were used in duplex PCR for B.
bigemina and T.
evansi amplified products of 689 bp and 257 bp, respectively.
The overall prevalence by duplex PCR was found to be 36.49, 2.43, and 3.41% for T.
evansi, B.
bigemina, and dual infection, respectively.
A more significant difference was observed for dual infection status (P≤0.005) as compared to T.
evansi (P≤0.05) and B.
bigemina (P≤0.01) among various districts under study.
A very low prevalence of T.
evansi (0.73%) and B.
bigemina (0.48%) was seen by GSTBS.
The highly sensitive, specific, and cost-effective duplex PCR was able to detect latent T.
evansi and B.
bigemina infection in cattle and buffaloes.
Haematological evaluation revealed marked pathology in B.
bigemina infected group and in dual infected group in contrast to that infected with T.
evansi alone.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sharma, Amrita& Singla, Lachhman Das& Tuli, Ashuma& Kaur, Paramjit& Batth, Balwinder Kaur& Javed, Mohammed…[et al.]. 2013. Molecular Prevalence of Babesia bigemina and Trypanosoma evansi in Dairy Animals from Punjab, India, by Duplex PCR: A Step Forward to the Detection and Management of Concurrent Latent Infections. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005344
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sharma, Amrita…[et al.]. Molecular Prevalence of Babesia bigemina and Trypanosoma evansi in Dairy Animals from Punjab, India, by Duplex PCR: A Step Forward to the Detection and Management of Concurrent Latent Infections. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1005344
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sharma, Amrita& Singla, Lachhman Das& Tuli, Ashuma& Kaur, Paramjit& Batth, Balwinder Kaur& Javed, Mohammed…[et al.]. Molecular Prevalence of Babesia bigemina and Trypanosoma evansi in Dairy Animals from Punjab, India, by Duplex PCR: A Step Forward to the Detection and Management of Concurrent Latent Infections. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005344
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1005344
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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