Molecular Prevalence of Babesia bigemina and Trypanosoma evansi in Dairy Animals from Punjab, India, by Duplex PCR: A Step Forward to the Detection and Management of Concurrent Latent Infections
Joint Authors
Sharma, Amrita
Singla, Lachhman Das
Tuli, Ashuma
Kaur, Paramjit
Batth, Balwinder Kaur
Javed, Mohammed
Juyal, Prayag Dutt
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-29
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Specific duplex polymerase chain reaction (PCR) was employed on 411 (386 cattle and 25 buffaloes) blood samples of dairy animals from 9 districts of Punjab, India, for simultaneous detection of Babesia bigemina and Trypanosoma evansi.
The results were compared and correlated with conventional Giemsa stained thin blood smear (GSTBS) examination and haematological alterations to know the clinical status and pathogenicity of infections.
The Bg3/Bg4 and TR3/TR4 primers were used in duplex PCR for B.
bigemina and T.
evansi amplified products of 689 bp and 257 bp, respectively.
The overall prevalence by duplex PCR was found to be 36.49, 2.43, and 3.41% for T.
evansi, B.
bigemina, and dual infection, respectively.
A more significant difference was observed for dual infection status (P≤0.005) as compared to T.
evansi (P≤0.05) and B.
bigemina (P≤0.01) among various districts under study.
A very low prevalence of T.
evansi (0.73%) and B.
bigemina (0.48%) was seen by GSTBS.
The highly sensitive, specific, and cost-effective duplex PCR was able to detect latent T.
evansi and B.
bigemina infection in cattle and buffaloes.
Haematological evaluation revealed marked pathology in B.
bigemina infected group and in dual infected group in contrast to that infected with T.
evansi alone.
American Psychological Association (APA)
Sharma, Amrita& Singla, Lachhman Das& Tuli, Ashuma& Kaur, Paramjit& Batth, Balwinder Kaur& Javed, Mohammed…[et al.]. 2013. Molecular Prevalence of Babesia bigemina and Trypanosoma evansi in Dairy Animals from Punjab, India, by Duplex PCR: A Step Forward to the Detection and Management of Concurrent Latent Infections. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005344
Modern Language Association (MLA)
Sharma, Amrita…[et al.]. Molecular Prevalence of Babesia bigemina and Trypanosoma evansi in Dairy Animals from Punjab, India, by Duplex PCR: A Step Forward to the Detection and Management of Concurrent Latent Infections. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1005344
American Medical Association (AMA)
Sharma, Amrita& Singla, Lachhman Das& Tuli, Ashuma& Kaur, Paramjit& Batth, Balwinder Kaur& Javed, Mohammed…[et al.]. Molecular Prevalence of Babesia bigemina and Trypanosoma evansi in Dairy Animals from Punjab, India, by Duplex PCR: A Step Forward to the Detection and Management of Concurrent Latent Infections. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005344
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005344