Emerging Rapid Resistance Testing Methods for Clinical Microbiology Laboratories and Their Potential Impact on Patient Management
المؤلفون المشاركون
Masanta, Wycliffe Omurwa
Zautner, Andreas E.
Frickmann, Hagen
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-19، 19ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-09-17
دولة النشر
مصر
عدد الصفحات
19
التخصصات الرئيسية
الملخص EN
Atypical and multidrug resistance, especially ESBL and carbapenemase expressing Enterobacteriaceae, is globally spreading.
Therefore, it becomes increasingly difficult to achieve therapeutic success by calculated antibiotic therapy.
Consequently, rapid antibiotic resistance testing is essential.
Various molecular and mass spectrometry-based approaches have been introduced in diagnostic microbiology to speed up the providing of reliable resistance data.
PCR- and sequencing-based approaches are the most expensive but the most frequently applied modes of testing, suitable for the detection of resistance genes even from primary material.
Next generation sequencing, based either on assessment of allelic single nucleotide polymorphisms or on the detection of nonubiquitous resistance mechanisms might allow for sequence-based bacterial resistance testing comparable to viral resistance testing on the long term.
Fluorescence in situ hybridization (FISH), based on specific binding of fluorescence-labeled oligonucleotide probes, provides a less expensive molecular bridging technique.
It is particularly useful for detection of resistance mechanisms based on mutations in ribosomal RNA.
Approaches based on MALDI-TOF-MS, alone or in combination with molecular techniques, like PCR/electrospray ionization MS or minisequencing provide the fastest resistance results from pure colonies or even primary samples with a growing number of protocols.
This review details the various approaches of rapid resistance testing, their pros and cons, and their potential use for the diagnostic laboratory.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Frickmann, Hagen& Masanta, Wycliffe Omurwa& Zautner, Andreas E.. 2014. Emerging Rapid Resistance Testing Methods for Clinical Microbiology Laboratories and Their Potential Impact on Patient Management. BioMed Research International،Vol. 2014, no. 2014, pp.1-19.
https://search.emarefa.net/detail/BIM-1016257
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Frickmann, Hagen…[et al.]. Emerging Rapid Resistance Testing Methods for Clinical Microbiology Laboratories and Their Potential Impact on Patient Management. BioMed Research International No. 2014 (2014), pp.1-19.
https://search.emarefa.net/detail/BIM-1016257
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Frickmann, Hagen& Masanta, Wycliffe Omurwa& Zautner, Andreas E.. Emerging Rapid Resistance Testing Methods for Clinical Microbiology Laboratories and Their Potential Impact on Patient Management. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-19.
https://search.emarefa.net/detail/BIM-1016257
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1016257
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر