![](/images/graphics-bg.png)
Emerging Rapid Resistance Testing Methods for Clinical Microbiology Laboratories and Their Potential Impact on Patient Management
Joint Authors
Masanta, Wycliffe Omurwa
Zautner, Andreas E.
Frickmann, Hagen
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-17
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Atypical and multidrug resistance, especially ESBL and carbapenemase expressing Enterobacteriaceae, is globally spreading.
Therefore, it becomes increasingly difficult to achieve therapeutic success by calculated antibiotic therapy.
Consequently, rapid antibiotic resistance testing is essential.
Various molecular and mass spectrometry-based approaches have been introduced in diagnostic microbiology to speed up the providing of reliable resistance data.
PCR- and sequencing-based approaches are the most expensive but the most frequently applied modes of testing, suitable for the detection of resistance genes even from primary material.
Next generation sequencing, based either on assessment of allelic single nucleotide polymorphisms or on the detection of nonubiquitous resistance mechanisms might allow for sequence-based bacterial resistance testing comparable to viral resistance testing on the long term.
Fluorescence in situ hybridization (FISH), based on specific binding of fluorescence-labeled oligonucleotide probes, provides a less expensive molecular bridging technique.
It is particularly useful for detection of resistance mechanisms based on mutations in ribosomal RNA.
Approaches based on MALDI-TOF-MS, alone or in combination with molecular techniques, like PCR/electrospray ionization MS or minisequencing provide the fastest resistance results from pure colonies or even primary samples with a growing number of protocols.
This review details the various approaches of rapid resistance testing, their pros and cons, and their potential use for the diagnostic laboratory.
American Psychological Association (APA)
Frickmann, Hagen& Masanta, Wycliffe Omurwa& Zautner, Andreas E.. 2014. Emerging Rapid Resistance Testing Methods for Clinical Microbiology Laboratories and Their Potential Impact on Patient Management. BioMed Research International،Vol. 2014, no. 2014, pp.1-19.
https://search.emarefa.net/detail/BIM-1016257
Modern Language Association (MLA)
Frickmann, Hagen…[et al.]. Emerging Rapid Resistance Testing Methods for Clinical Microbiology Laboratories and Their Potential Impact on Patient Management. BioMed Research International No. 2014 (2014), pp.1-19.
https://search.emarefa.net/detail/BIM-1016257
American Medical Association (AMA)
Frickmann, Hagen& Masanta, Wycliffe Omurwa& Zautner, Andreas E.. Emerging Rapid Resistance Testing Methods for Clinical Microbiology Laboratories and Their Potential Impact on Patient Management. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-19.
https://search.emarefa.net/detail/BIM-1016257
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016257