Angiotensin I Converting Enzyme Inhibitory Peptides Obtained after In Vitro Hydrolysis of Pea (Pisum sativum var. Bajka) Globulins
المؤلفون المشاركون
Jakubczyk, Anna
Baraniak, Barbara
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-27
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Pea seeds represent a valuable source of active compounds that may positively influence health.
In this study, the pea globulins were digested in vitro under gastrointestinal condition and potentially bioaccessible angiotensin I converting enzyme (ACE) inhibitory peptides were identified.
The degree of hydrolysis after pepsin, 14.42%, and pancreatin, 30.65%, were noted.
The peptides with the highest ACE inhibitory properties were separated using ion exchange chromatography on DEAE-cellulose.
Thirteen peptides fractions were obtained but only four showed potential antihypertensive properties.
The highest inhibitory activity was determined for the fraction F8 (IC50 = 0.0014 mg/mL).
This fraction was separated on Sephadex G10 and two peptide fractions were obtained.
The peptides fraction (B) with the highest ACE inhibitory activity (IC50 = 0.073 mg/mL) was identified by ESI-MS/MS.
The sequences of ACE inhibitory peptides were GGSGNY, DLKLP, GSSDNR, MRDLK, and HNTPSR.
Based on Lineweaver-Burk plots for the fraction B, the kinetic parameters as Km, Vmax, and Ki and mode of inhibition were determined.
This fraction belongs to uncompetitive inhibitor of ACE activity.
The seeds of pea are the source of precursor protein, which releases the ACE inhibitory peptides as a result of enzymatic hydrolysis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jakubczyk, Anna& Baraniak, Barbara. 2014. Angiotensin I Converting Enzyme Inhibitory Peptides Obtained after In Vitro Hydrolysis of Pea (Pisum sativum var. Bajka) Globulins. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016293
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jakubczyk, Anna& Baraniak, Barbara. Angiotensin I Converting Enzyme Inhibitory Peptides Obtained after In Vitro Hydrolysis of Pea (Pisum sativum var. Bajka) Globulins. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1016293
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jakubczyk, Anna& Baraniak, Barbara. Angiotensin I Converting Enzyme Inhibitory Peptides Obtained after In Vitro Hydrolysis of Pea (Pisum sativum var. Bajka) Globulins. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016293
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1016293
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر