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Angiotensin I Converting Enzyme Inhibitory Peptides Obtained after In Vitro Hydrolysis of Pea (Pisum sativum var. Bajka) Globulins
Joint Authors
Jakubczyk, Anna
Baraniak, Barbara
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Pea seeds represent a valuable source of active compounds that may positively influence health.
In this study, the pea globulins were digested in vitro under gastrointestinal condition and potentially bioaccessible angiotensin I converting enzyme (ACE) inhibitory peptides were identified.
The degree of hydrolysis after pepsin, 14.42%, and pancreatin, 30.65%, were noted.
The peptides with the highest ACE inhibitory properties were separated using ion exchange chromatography on DEAE-cellulose.
Thirteen peptides fractions were obtained but only four showed potential antihypertensive properties.
The highest inhibitory activity was determined for the fraction F8 (IC50 = 0.0014 mg/mL).
This fraction was separated on Sephadex G10 and two peptide fractions were obtained.
The peptides fraction (B) with the highest ACE inhibitory activity (IC50 = 0.073 mg/mL) was identified by ESI-MS/MS.
The sequences of ACE inhibitory peptides were GGSGNY, DLKLP, GSSDNR, MRDLK, and HNTPSR.
Based on Lineweaver-Burk plots for the fraction B, the kinetic parameters as Km, Vmax, and Ki and mode of inhibition were determined.
This fraction belongs to uncompetitive inhibitor of ACE activity.
The seeds of pea are the source of precursor protein, which releases the ACE inhibitory peptides as a result of enzymatic hydrolysis.
American Psychological Association (APA)
Jakubczyk, Anna& Baraniak, Barbara. 2014. Angiotensin I Converting Enzyme Inhibitory Peptides Obtained after In Vitro Hydrolysis of Pea (Pisum sativum var. Bajka) Globulins. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016293
Modern Language Association (MLA)
Jakubczyk, Anna& Baraniak, Barbara. Angiotensin I Converting Enzyme Inhibitory Peptides Obtained after In Vitro Hydrolysis of Pea (Pisum sativum var. Bajka) Globulins. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1016293
American Medical Association (AMA)
Jakubczyk, Anna& Baraniak, Barbara. Angiotensin I Converting Enzyme Inhibitory Peptides Obtained after In Vitro Hydrolysis of Pea (Pisum sativum var. Bajka) Globulins. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016293
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016293