Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro
المؤلفون المشاركون
Huang, Di
Yang, Lubing
Wang, Chenlu
Ma, Sihui
Cui, Li
Huang, Shiyang
Sheng, Xia
Weng, Qiang
Xu, Meiyu
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-12-21
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Oviductus Ranae is the dry oviduct of Rana chensinensis, which is also called R.
chensinensis oil.
Oviductus Ranae is a valuable Chinese crude drug and is recorded in the Pharmacopoeia of the People’s Republic of China.
The aim of this study was to investigate the immunostimulatory activity of protein hydrolysate of Oviductus Ranae (ORPH) and to assess its possible mechanism.
Immunomodulatory activity of ORPH was examined in murine macrophage RAW 264.7 cells.
The effect of ORPH on the phagocytic activity of macrophages was determined by the neutral red uptake assay.
After treatment with ORPH, NO production levels in the culture supernatant were investigated by Griess assay.
The mRNA and protein expressions of inducible nitric oxide synthase (iNOS) were detected by RT-PCR and Western blotting.
The production of TNF-α, IL-1β, and IL-6 after treatment with ORPH was measured using ELISA assay.
In addition, NF-κB levels were also investigated by Western blot.
The results showed that ORPH enhanced the phagocytosis of macrophage, increased productions of TNF-α, IL-1β, IL-6, and NO in RAW 264.7 cells, and upregulated the mRNA and protein expression of iNOS.
Besides, NF-κB, levels in RAW 264.7 cells were elevated after ORPH treatment.
These findings suggested that ORPH might stimulate macrophage activities by activating the NF-κB pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Huang, Di& Yang, Lubing& Wang, Chenlu& Ma, Sihui& Cui, Li& Huang, Shiyang…[et al.]. 2014. Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1018183
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Huang, Di…[et al.]. Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1018183
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Huang, Di& Yang, Lubing& Wang, Chenlu& Ma, Sihui& Cui, Li& Huang, Shiyang…[et al.]. Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1018183
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1018183
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر