Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro
Joint Authors
Huang, Di
Yang, Lubing
Wang, Chenlu
Ma, Sihui
Cui, Li
Huang, Shiyang
Sheng, Xia
Weng, Qiang
Xu, Meiyu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-21
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Oviductus Ranae is the dry oviduct of Rana chensinensis, which is also called R.
chensinensis oil.
Oviductus Ranae is a valuable Chinese crude drug and is recorded in the Pharmacopoeia of the People’s Republic of China.
The aim of this study was to investigate the immunostimulatory activity of protein hydrolysate of Oviductus Ranae (ORPH) and to assess its possible mechanism.
Immunomodulatory activity of ORPH was examined in murine macrophage RAW 264.7 cells.
The effect of ORPH on the phagocytic activity of macrophages was determined by the neutral red uptake assay.
After treatment with ORPH, NO production levels in the culture supernatant were investigated by Griess assay.
The mRNA and protein expressions of inducible nitric oxide synthase (iNOS) were detected by RT-PCR and Western blotting.
The production of TNF-α, IL-1β, and IL-6 after treatment with ORPH was measured using ELISA assay.
In addition, NF-κB levels were also investigated by Western blot.
The results showed that ORPH enhanced the phagocytosis of macrophage, increased productions of TNF-α, IL-1β, IL-6, and NO in RAW 264.7 cells, and upregulated the mRNA and protein expression of iNOS.
Besides, NF-κB, levels in RAW 264.7 cells were elevated after ORPH treatment.
These findings suggested that ORPH might stimulate macrophage activities by activating the NF-κB pathway.
American Psychological Association (APA)
Huang, Di& Yang, Lubing& Wang, Chenlu& Ma, Sihui& Cui, Li& Huang, Shiyang…[et al.]. 2014. Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1018183
Modern Language Association (MLA)
Huang, Di…[et al.]. Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1018183
American Medical Association (AMA)
Huang, Di& Yang, Lubing& Wang, Chenlu& Ma, Sihui& Cui, Li& Huang, Shiyang…[et al.]. Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1018183
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018183