Evaluation of the available anti-HCV antibody Detection Tests and RT-PCR assay in the Diagnosis of Hepatitis C Virus Infection
المؤلفون المشاركون
Gazzaz, Zuhayr J.
Dhaffar, Khalid O.
Khudari, Yusuf A.
Ibrahim, Ayidah Muhammad
Azab, Badr A.
Tashkandi, Muhammad Amin
المصدر
Saudi Journal of Kidney Diseases and Transplantation
العدد
المجلد 18، العدد 4 (31 ديسمبر/كانون الأول 2007)، ص ص. 523-531، 9ص.
الناشر
تاريخ النشر
2007-12-31
دولة النشر
السعودية
عدد الصفحات
9
التخصصات الرئيسية
الموضوعات
الملخص EN
The aim of the present work is to evaluate the commercially available antibody tests in the diagnosis of hepatitis C virus (HCV) infection by comparing their results with the RT-PCR test.
The study included 316 serum samples from three groups : blood donors, patients on maintenance hemodialysis (HD) and patients infected with the human immunodeficiency virus (HIV).
Samples were subjected to HCV-antibody detection by ELISA and RIBA tests and HCVRNA detection by RT-PCR assay.
The percentage of infectivity for blood donors was 18.9 % by ELISA, 20.8 % by RIBA and 23.6 % by RT-PCR test.
For patients on HD and those positive for HIV, the test positivity was respectively 59.3 % and 5.3 % by ELISA, 64 % and 10.5 % by RIBA and, 66.3 % and 21 % by PCR test.
The percentage of false negativity of HCV-Ab by ELISA and RIBA when compared with RT-PCR test was 3.5 and 8.1 % for samples blood donors, 17.1 and 25.7 % for HD patients and 5.6 and 16.7 % for HIV-infected samples, respectively.
The false positivity of HCV-Ab by ELISA and RIBA, when compared with RT-PCR, was 5 %, 3.9 % and zero for blood donors, HD patients and HIV-HCV co-infected cases, respectively.
While comparing ELISA with RT-PCR, the false positivity was 10 %, 5.9 % and zero respectively for blood donors, HD patients and HIV-HCV co-infected cases.
Thus, it is very important to screen blood donors, HD patients and HIV-infected patients by using the RT-PCR for HCV-RNA to avoid false negative results.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tashkandi, Muhammad Amin& Khudari, Yusuf A.& Ibrahim, Ayidah Muhammad& Dhaffar, Khalid O.& Gazzaz, Zuhayr J.& Azab, Badr A.. 2007. Evaluation of the available anti-HCV antibody Detection Tests and RT-PCR assay in the Diagnosis of Hepatitis C Virus Infection. Saudi Journal of Kidney Diseases and Transplantation،Vol. 18, no. 4, pp.523-531.
https://search.emarefa.net/detail/BIM-102588
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tashkandi, Muhammad Amin…[et al.]. Evaluation of the available anti-HCV antibody Detection Tests and RT-PCR assay in the Diagnosis of Hepatitis C Virus Infection. Saudi Journal of Kidney Diseases and Transplantation Vol. 18, no. 4 (Dec. 2007), pp.523-531.
https://search.emarefa.net/detail/BIM-102588
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tashkandi, Muhammad Amin& Khudari, Yusuf A.& Ibrahim, Ayidah Muhammad& Dhaffar, Khalid O.& Gazzaz, Zuhayr J.& Azab, Badr A.. Evaluation of the available anti-HCV antibody Detection Tests and RT-PCR assay in the Diagnosis of Hepatitis C Virus Infection. Saudi Journal of Kidney Diseases and Transplantation. 2007. Vol. 18, no. 4, pp.523-531.
https://search.emarefa.net/detail/BIM-102588
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 530-531
رقم السجل
BIM-102588
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر