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Evaluation of the available anti-HCV antibody Detection Tests and RT-PCR assay in the Diagnosis of Hepatitis C Virus Infection
Joint Authors
Gazzaz, Zuhayr J.
Dhaffar, Khalid O.
Khudari, Yusuf A.
Ibrahim, Ayidah Muhammad
Azab, Badr A.
Tashkandi, Muhammad Amin
Source
Saudi Journal of Kidney Diseases and Transplantation
Issue
Vol. 18, Issue 4 (31 Dec. 2007), pp.523-531, 9 p.
Publisher
Saudi Center for Organ Transplantation
Publication Date
2007-12-31
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Topics
Abstract EN
The aim of the present work is to evaluate the commercially available antibody tests in the diagnosis of hepatitis C virus (HCV) infection by comparing their results with the RT-PCR test.
The study included 316 serum samples from three groups : blood donors, patients on maintenance hemodialysis (HD) and patients infected with the human immunodeficiency virus (HIV).
Samples were subjected to HCV-antibody detection by ELISA and RIBA tests and HCVRNA detection by RT-PCR assay.
The percentage of infectivity for blood donors was 18.9 % by ELISA, 20.8 % by RIBA and 23.6 % by RT-PCR test.
For patients on HD and those positive for HIV, the test positivity was respectively 59.3 % and 5.3 % by ELISA, 64 % and 10.5 % by RIBA and, 66.3 % and 21 % by PCR test.
The percentage of false negativity of HCV-Ab by ELISA and RIBA when compared with RT-PCR test was 3.5 and 8.1 % for samples blood donors, 17.1 and 25.7 % for HD patients and 5.6 and 16.7 % for HIV-infected samples, respectively.
The false positivity of HCV-Ab by ELISA and RIBA, when compared with RT-PCR, was 5 %, 3.9 % and zero for blood donors, HD patients and HIV-HCV co-infected cases, respectively.
While comparing ELISA with RT-PCR, the false positivity was 10 %, 5.9 % and zero respectively for blood donors, HD patients and HIV-HCV co-infected cases.
Thus, it is very important to screen blood donors, HD patients and HIV-infected patients by using the RT-PCR for HCV-RNA to avoid false negative results.
American Psychological Association (APA)
Tashkandi, Muhammad Amin& Khudari, Yusuf A.& Ibrahim, Ayidah Muhammad& Dhaffar, Khalid O.& Gazzaz, Zuhayr J.& Azab, Badr A.. 2007. Evaluation of the available anti-HCV antibody Detection Tests and RT-PCR assay in the Diagnosis of Hepatitis C Virus Infection. Saudi Journal of Kidney Diseases and Transplantation،Vol. 18, no. 4, pp.523-531.
https://search.emarefa.net/detail/BIM-102588
Modern Language Association (MLA)
Tashkandi, Muhammad Amin…[et al.]. Evaluation of the available anti-HCV antibody Detection Tests and RT-PCR assay in the Diagnosis of Hepatitis C Virus Infection. Saudi Journal of Kidney Diseases and Transplantation Vol. 18, no. 4 (Dec. 2007), pp.523-531.
https://search.emarefa.net/detail/BIM-102588
American Medical Association (AMA)
Tashkandi, Muhammad Amin& Khudari, Yusuf A.& Ibrahim, Ayidah Muhammad& Dhaffar, Khalid O.& Gazzaz, Zuhayr J.& Azab, Badr A.. Evaluation of the available anti-HCV antibody Detection Tests and RT-PCR assay in the Diagnosis of Hepatitis C Virus Infection. Saudi Journal of Kidney Diseases and Transplantation. 2007. Vol. 18, no. 4, pp.523-531.
https://search.emarefa.net/detail/BIM-102588
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 530-531
Record ID
BIM-102588