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New Role for L-Arginine in Regulation of Inducible Nitric-Oxide-Synthase-Derived Superoxide Anion Production in Raw 264.7 Macrophages
المؤلفون المشاركون
Vasicek, Ondrej
Pekarova, Michaela
Martiskova, Hana
Bino, Lucia
Klinke, A.
Lau, D.
Kuchta, Radek
Kadlec, Jaroslav
Vrba, Radimir
Lojek, Antonin
Kubala, Lukas
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-12-18
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
Dietary supplementation with L-arginine was shown to improve immune responses in various inflammatory models.
However, the molecular mechanisms underlying L-arginine effects on immune cells remain unrecognized.
Herein, we tested the hypothesis that a limitation of L-arginine could lead to the uncoupled state of murine macrophage inducible nitric oxide synthase and, therefore, increase inducible nitric-oxide-synthase-derived superoxide anion formation.
Importantly, we demonstrated that L-arginine dose- and time dependently potentiated superoxide anion production in bacterial endotoxin-stimulated macrophages, although it did not influence NADPH oxidase expression and activity.
Detailed analysis of macrophage activation showed the time dependence between LPS-induced iNOS expression and increased O2∙- formation.
Moreover, downregulation of macrophage iNOS expression, as well as the inhibition of iNOS activity by NOS inhibitors, unveiled an important role of this enzyme in controlling O2∙- and peroxynitrite formation during macrophage stimulation.
In conclusion, our data demonstrated that simultaneous induction of NADPH oxidase, together with the iNOS enzyme, can result in the uncoupled state of iNOS resulting in the production of functionally important levels of O2∙- soon after macrophage activation with LPS.
Moreover, we demonstrated, for the first time that increased concentrations of L-arginine further potentiate iNOS-dependent O2∙- formation in inflammatory macrophages.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pekarova, Michaela& Lojek, Antonin& Martiskova, Hana& Vasicek, Ondrej& Bino, Lucia& Klinke, A.…[et al.]. 2011. New Role for L-Arginine in Regulation of Inducible Nitric-Oxide-Synthase-Derived Superoxide Anion Production in Raw 264.7 Macrophages. The Scientific World Journal،Vol. 2011, no. 2011, pp.1-15.
https://search.emarefa.net/detail/BIM-1027867
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pekarova, Michaela…[et al.]. New Role for L-Arginine in Regulation of Inducible Nitric-Oxide-Synthase-Derived Superoxide Anion Production in Raw 264.7 Macrophages. The Scientific World Journal No. 2011 (2011), pp.1-15.
https://search.emarefa.net/detail/BIM-1027867
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pekarova, Michaela& Lojek, Antonin& Martiskova, Hana& Vasicek, Ondrej& Bino, Lucia& Klinke, A.…[et al.]. New Role for L-Arginine in Regulation of Inducible Nitric-Oxide-Synthase-Derived Superoxide Anion Production in Raw 264.7 Macrophages. The Scientific World Journal. 2011. Vol. 2011, no. 2011, pp.1-15.
https://search.emarefa.net/detail/BIM-1027867
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1027867
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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