New Role for L-Arginine in Regulation of Inducible Nitric-Oxide-Synthase-Derived Superoxide Anion Production in Raw 264.7 Macrophages
Joint Authors
Vasicek, Ondrej
Pekarova, Michaela
Martiskova, Hana
Bino, Lucia
Klinke, A.
Lau, D.
Kuchta, Radek
Kadlec, Jaroslav
Vrba, Radimir
Lojek, Antonin
Kubala, Lukas
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-18
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Dietary supplementation with L-arginine was shown to improve immune responses in various inflammatory models.
However, the molecular mechanisms underlying L-arginine effects on immune cells remain unrecognized.
Herein, we tested the hypothesis that a limitation of L-arginine could lead to the uncoupled state of murine macrophage inducible nitric oxide synthase and, therefore, increase inducible nitric-oxide-synthase-derived superoxide anion formation.
Importantly, we demonstrated that L-arginine dose- and time dependently potentiated superoxide anion production in bacterial endotoxin-stimulated macrophages, although it did not influence NADPH oxidase expression and activity.
Detailed analysis of macrophage activation showed the time dependence between LPS-induced iNOS expression and increased O2∙- formation.
Moreover, downregulation of macrophage iNOS expression, as well as the inhibition of iNOS activity by NOS inhibitors, unveiled an important role of this enzyme in controlling O2∙- and peroxynitrite formation during macrophage stimulation.
In conclusion, our data demonstrated that simultaneous induction of NADPH oxidase, together with the iNOS enzyme, can result in the uncoupled state of iNOS resulting in the production of functionally important levels of O2∙- soon after macrophage activation with LPS.
Moreover, we demonstrated, for the first time that increased concentrations of L-arginine further potentiate iNOS-dependent O2∙- formation in inflammatory macrophages.
American Psychological Association (APA)
Pekarova, Michaela& Lojek, Antonin& Martiskova, Hana& Vasicek, Ondrej& Bino, Lucia& Klinke, A.…[et al.]. 2011. New Role for L-Arginine in Regulation of Inducible Nitric-Oxide-Synthase-Derived Superoxide Anion Production in Raw 264.7 Macrophages. The Scientific World Journal،Vol. 2011, no. 2011, pp.1-15.
https://search.emarefa.net/detail/BIM-1027867
Modern Language Association (MLA)
Pekarova, Michaela…[et al.]. New Role for L-Arginine in Regulation of Inducible Nitric-Oxide-Synthase-Derived Superoxide Anion Production in Raw 264.7 Macrophages. The Scientific World Journal No. 2011 (2011), pp.1-15.
https://search.emarefa.net/detail/BIM-1027867
American Medical Association (AMA)
Pekarova, Michaela& Lojek, Antonin& Martiskova, Hana& Vasicek, Ondrej& Bino, Lucia& Klinke, A.…[et al.]. New Role for L-Arginine in Regulation of Inducible Nitric-Oxide-Synthase-Derived Superoxide Anion Production in Raw 264.7 Macrophages. The Scientific World Journal. 2011. Vol. 2011, no. 2011, pp.1-15.
https://search.emarefa.net/detail/BIM-1027867
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1027867