Mitigation of H2O2-Induced Mitochondrial-Mediated Apoptosis in NG108-15 Cells by Novel Mesuagenin C from Mesua kunstleri (King) Kosterm
المؤلفون المشاركون
Abdul Kadir, Habsah
Chan, Gomathi
Kamarudin, Muhamad Noor Alfarizal
Wong, Daniel Zin Hua
Ismail, Nor Hadiani
Abdul Latif, Faizuri
Hasan, Aurengzeb
Awang, Khalijah
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-18، 18ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-05-07
دولة النشر
مصر
عدد الصفحات
18
التخصصات الرئيسية
الملخص EN
This study was aimed to isolate and evaluate neuroprotective compounds from the hexane extract of the bark of Mesua kunstleri (Clusiaceae) on H2O2-induced apoptosis in NG108-15 cells.
Five 4-phenylcoumarins were isolated by using various chromatographic techniques via neuroprotective activity-guided fractionation and isolation from the active hexane extract.
The chemical structures of the isolated compounds were confirmed by NMR spectroscopic data interpretation and comparison with literature values.
Cell viability data demonstrated that mesuagenin C 3 significantly increased cell viability.
Hoechst 33342/PI staining illustrated mesuagenin C 3 was able to abate the nuclear shrinkage, chromatin condensation and formation of apoptotic bodies.
Pretreatment with mesuagenin C 3 reduced total annexin V positive cells and increased the level of intracellular glutathione (GSH).
Mesuagenin C 3 attenuated membrane potential (Δψm), reduced Bax/Bcl-2 ratio and inactivated of caspase-3/7 and -9.
These results indicated that mesuagenin C 3 could protect NG108-15 cells against H2O2-induced apoptosis by increasing intracellular GSH level, aggrandizing Δψm, and modulating apoptotic signalling pathway through Bcl-2 family and caspase-3/7 and -9.
These findings confirmed the involvement of intrinsic apoptotic pathway in H2O2-induced apoptosis and suggested that mesuagenin C 3 may have potential therapeutic properties for neurodegenerative diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chan, Gomathi& Kamarudin, Muhamad Noor Alfarizal& Wong, Daniel Zin Hua& Ismail, Nor Hadiani& Abdul Latif, Faizuri& Hasan, Aurengzeb…[et al.]. 2012. Mitigation of H2O2-Induced Mitochondrial-Mediated Apoptosis in NG108-15 Cells by Novel Mesuagenin C from Mesua kunstleri (King) Kosterm. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-18.
https://search.emarefa.net/detail/BIM-1028124
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chan, Gomathi…[et al.]. Mitigation of H2O2-Induced Mitochondrial-Mediated Apoptosis in NG108-15 Cells by Novel Mesuagenin C from Mesua kunstleri (King) Kosterm. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-18.
https://search.emarefa.net/detail/BIM-1028124
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chan, Gomathi& Kamarudin, Muhamad Noor Alfarizal& Wong, Daniel Zin Hua& Ismail, Nor Hadiani& Abdul Latif, Faizuri& Hasan, Aurengzeb…[et al.]. Mitigation of H2O2-Induced Mitochondrial-Mediated Apoptosis in NG108-15 Cells by Novel Mesuagenin C from Mesua kunstleri (King) Kosterm. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-18.
https://search.emarefa.net/detail/BIM-1028124
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1028124
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر