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Mitigation of H2O2-Induced Mitochondrial-Mediated Apoptosis in NG108-15 Cells by Novel Mesuagenin C from Mesua kunstleri (King) Kosterm
Joint Authors
Abdul Kadir, Habsah
Chan, Gomathi
Kamarudin, Muhamad Noor Alfarizal
Wong, Daniel Zin Hua
Ismail, Nor Hadiani
Abdul Latif, Faizuri
Hasan, Aurengzeb
Awang, Khalijah
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-07
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
This study was aimed to isolate and evaluate neuroprotective compounds from the hexane extract of the bark of Mesua kunstleri (Clusiaceae) on H2O2-induced apoptosis in NG108-15 cells.
Five 4-phenylcoumarins were isolated by using various chromatographic techniques via neuroprotective activity-guided fractionation and isolation from the active hexane extract.
The chemical structures of the isolated compounds were confirmed by NMR spectroscopic data interpretation and comparison with literature values.
Cell viability data demonstrated that mesuagenin C 3 significantly increased cell viability.
Hoechst 33342/PI staining illustrated mesuagenin C 3 was able to abate the nuclear shrinkage, chromatin condensation and formation of apoptotic bodies.
Pretreatment with mesuagenin C 3 reduced total annexin V positive cells and increased the level of intracellular glutathione (GSH).
Mesuagenin C 3 attenuated membrane potential (Δψm), reduced Bax/Bcl-2 ratio and inactivated of caspase-3/7 and -9.
These results indicated that mesuagenin C 3 could protect NG108-15 cells against H2O2-induced apoptosis by increasing intracellular GSH level, aggrandizing Δψm, and modulating apoptotic signalling pathway through Bcl-2 family and caspase-3/7 and -9.
These findings confirmed the involvement of intrinsic apoptotic pathway in H2O2-induced apoptosis and suggested that mesuagenin C 3 may have potential therapeutic properties for neurodegenerative diseases.
American Psychological Association (APA)
Chan, Gomathi& Kamarudin, Muhamad Noor Alfarizal& Wong, Daniel Zin Hua& Ismail, Nor Hadiani& Abdul Latif, Faizuri& Hasan, Aurengzeb…[et al.]. 2012. Mitigation of H2O2-Induced Mitochondrial-Mediated Apoptosis in NG108-15 Cells by Novel Mesuagenin C from Mesua kunstleri (King) Kosterm. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-18.
https://search.emarefa.net/detail/BIM-1028124
Modern Language Association (MLA)
Chan, Gomathi…[et al.]. Mitigation of H2O2-Induced Mitochondrial-Mediated Apoptosis in NG108-15 Cells by Novel Mesuagenin C from Mesua kunstleri (King) Kosterm. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-18.
https://search.emarefa.net/detail/BIM-1028124
American Medical Association (AMA)
Chan, Gomathi& Kamarudin, Muhamad Noor Alfarizal& Wong, Daniel Zin Hua& Ismail, Nor Hadiani& Abdul Latif, Faizuri& Hasan, Aurengzeb…[et al.]. Mitigation of H2O2-Induced Mitochondrial-Mediated Apoptosis in NG108-15 Cells by Novel Mesuagenin C from Mesua kunstleri (King) Kosterm. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-18.
https://search.emarefa.net/detail/BIM-1028124
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028124