Triptolide Transcriptionally Represses HER2 in Ovarian Cancer Cells by Targeting NF-κB
المؤلفون المشاركون
Sytwu, Huey-Kang
Hsu, Shih-Chung
Ou, Chien-Chih
Chen, Yuan-Wu
Loh, Shih-Hurng
Li, Jhy-Wei
Liu, Jah-Yao
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-12-24
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Triptolide (TPL) inhibits the proliferation of a variety of cancer cells and has been proposed as an effective anticancer agent.
In this study, we demonstrate that TPL downregulates HER2 protein expression in oral, ovarian, and breast cancer cells.
It suppresses HER2 protein expression in a dose- and time-dependent manner.
Transrepression of HER2 promoter activity by TPL is also observed.
The interacting site of TPL on the HER2 promoter region is located between −207 and −103 bps, which includes a putative binding site for the transcription factor NF-κB.
Previous reports demonstrated that TPL suppresses NF-κB expression.
We demonstrate that overexpression of NF-κB rescues TPL-mediated suppression of HER2 promoter activity and protein expression in NIH3T3 cells and ovarian cancer cells, respectively.
In addition, TPL downregulates the activated (phosphorylated) forms of HER2, phosphoinositide-3 kinase (PI3K), and serine/threonine-specific protein kinase (Akt).
TPL also inhibits tumor growth in a mouse model.
Furthermore, TPL suppresses HER2 and Ki-67 expression in xenografted tumors based on an immunohistochemistry (IHC) assay.
These findings suggest that TPL transrepresses HER2 and suppresses the downstream PI3K/Akt-signaling pathway.
Our study reveals that TPL can inhibit tumor growth and thereby may serve as a potential chemotherapeutic agent.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ou, Chien-Chih& Chen, Yuan-Wu& Hsu, Shih-Chung& Sytwu, Huey-Kang& Loh, Shih-Hurng& Li, Jhy-Wei…[et al.]. 2012. Triptolide Transcriptionally Represses HER2 in Ovarian Cancer Cells by Targeting NF-κB. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1028264
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ou, Chien-Chih…[et al.]. Triptolide Transcriptionally Represses HER2 in Ovarian Cancer Cells by Targeting NF-κB. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-1028264
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ou, Chien-Chih& Chen, Yuan-Wu& Hsu, Shih-Chung& Sytwu, Huey-Kang& Loh, Shih-Hurng& Li, Jhy-Wei…[et al.]. Triptolide Transcriptionally Represses HER2 in Ovarian Cancer Cells by Targeting NF-κB. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1028264
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1028264
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر