Triptolide Transcriptionally Represses HER2 in Ovarian Cancer Cells by Targeting NF-κB
Joint Authors
Sytwu, Huey-Kang
Hsu, Shih-Chung
Ou, Chien-Chih
Chen, Yuan-Wu
Loh, Shih-Hurng
Li, Jhy-Wei
Liu, Jah-Yao
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Triptolide (TPL) inhibits the proliferation of a variety of cancer cells and has been proposed as an effective anticancer agent.
In this study, we demonstrate that TPL downregulates HER2 protein expression in oral, ovarian, and breast cancer cells.
It suppresses HER2 protein expression in a dose- and time-dependent manner.
Transrepression of HER2 promoter activity by TPL is also observed.
The interacting site of TPL on the HER2 promoter region is located between −207 and −103 bps, which includes a putative binding site for the transcription factor NF-κB.
Previous reports demonstrated that TPL suppresses NF-κB expression.
We demonstrate that overexpression of NF-κB rescues TPL-mediated suppression of HER2 promoter activity and protein expression in NIH3T3 cells and ovarian cancer cells, respectively.
In addition, TPL downregulates the activated (phosphorylated) forms of HER2, phosphoinositide-3 kinase (PI3K), and serine/threonine-specific protein kinase (Akt).
TPL also inhibits tumor growth in a mouse model.
Furthermore, TPL suppresses HER2 and Ki-67 expression in xenografted tumors based on an immunohistochemistry (IHC) assay.
These findings suggest that TPL transrepresses HER2 and suppresses the downstream PI3K/Akt-signaling pathway.
Our study reveals that TPL can inhibit tumor growth and thereby may serve as a potential chemotherapeutic agent.
American Psychological Association (APA)
Ou, Chien-Chih& Chen, Yuan-Wu& Hsu, Shih-Chung& Sytwu, Huey-Kang& Loh, Shih-Hurng& Li, Jhy-Wei…[et al.]. 2012. Triptolide Transcriptionally Represses HER2 in Ovarian Cancer Cells by Targeting NF-κB. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1028264
Modern Language Association (MLA)
Ou, Chien-Chih…[et al.]. Triptolide Transcriptionally Represses HER2 in Ovarian Cancer Cells by Targeting NF-κB. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-1028264
American Medical Association (AMA)
Ou, Chien-Chih& Chen, Yuan-Wu& Hsu, Shih-Chung& Sytwu, Huey-Kang& Loh, Shih-Hurng& Li, Jhy-Wei…[et al.]. Triptolide Transcriptionally Represses HER2 in Ovarian Cancer Cells by Targeting NF-κB. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1028264
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028264