Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation
المؤلفون المشاركون
Hassan, Loiy Elsir Ahmed
Abdul Majid, Amin M. S.
Yagi, Sakina M. Ahmed
Ahmad, Syahida
Chuen, Cheah Shiau
Narrima, Putri
Rais, Mohd Mustafa
Moharam, Bushra Abdulkarim
Hadi, A. Hamid A.
Abdel Wahab, Siddig Ibrahim
Syam, Suvitha
Mohan, Syam
Elhassan, Manal Mohamed
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-02-26
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Emerging evidence suggests that reactive oxygen (ROS) and nitrogen (RNS) species can contribute to diverse signalling pathways of inflammatory and tumour cells.
Cucurbitacins are a group of highly oxygenated triterpenes.
Many plants used in folk medicine to treat cancer have been found to contain cucurbitacins displaying potentially important anti-inflammatory actions.
The current study was designed to investigate the anti-ROS and -RNS effects of cucurbitacin L 2-O-β-glucoside (CLG) and the role of these signaling factors in the apoptogenic effects of CLG on human colon cancer cells (HT-29).
This natural cucurbitacin was isolated purely from Citrullus lanatus var.
citroides (Cucurbitaceae).
The results revealed that CLG was cytotoxic to HT-29.
CLG increased significantly (P<0.05) RNA and protein levels of caspase-3 in HT-29 cells when verified using a colorimetric assay and realtime qPCR, respectively.
The results showed that lipopolysaccharide/interferon-gamma (LPS/INF-γ) increased nitrous oxide (NO) production inR AW264.7macrophages, whereas N(G)-nitro-L-argininemethyl ester (L-NAME) and CLG curtailed it.
This compound did not reveal any cytotoxicity on RAW264.7 macrophages and human normal liver cells (WRL-68) when tested using the MTT assay.
Findings of ferric reducing antioxidant power (FRAP) and oxygen radical absorption capacity (ORAC) assays demonstrate the antioxidant properties of CLG.
The apoptogenic property of CLG on HT-29 cells is thus related to inhibition of reactive nitrogen and oxygen reactive species and the triggering of caspase-3-regulated apoptosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abdel Wahab, Siddig Ibrahim& Hassan, Loiy Elsir Ahmed& Abdul Majid, Amin M. S.& Yagi, Sakina M. Ahmed& Mohan, Syam& Elhassan, Manal Mohamed…[et al.]. 2012. Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1028348
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abdel Wahab, Siddig Ibrahim…[et al.]. Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-1028348
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abdel Wahab, Siddig Ibrahim& Hassan, Loiy Elsir Ahmed& Abdul Majid, Amin M. S.& Yagi, Sakina M. Ahmed& Mohan, Syam& Elhassan, Manal Mohamed…[et al.]. Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1028348
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1028348
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر