Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation
Joint Authors
Hassan, Loiy Elsir Ahmed
Abdul Majid, Amin M. S.
Yagi, Sakina M. Ahmed
Ahmad, Syahida
Chuen, Cheah Shiau
Narrima, Putri
Rais, Mohd Mustafa
Moharam, Bushra Abdulkarim
Hadi, A. Hamid A.
Abdel Wahab, Siddig Ibrahim
Syam, Suvitha
Mohan, Syam
Elhassan, Manal Mohamed
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Emerging evidence suggests that reactive oxygen (ROS) and nitrogen (RNS) species can contribute to diverse signalling pathways of inflammatory and tumour cells.
Cucurbitacins are a group of highly oxygenated triterpenes.
Many plants used in folk medicine to treat cancer have been found to contain cucurbitacins displaying potentially important anti-inflammatory actions.
The current study was designed to investigate the anti-ROS and -RNS effects of cucurbitacin L 2-O-β-glucoside (CLG) and the role of these signaling factors in the apoptogenic effects of CLG on human colon cancer cells (HT-29).
This natural cucurbitacin was isolated purely from Citrullus lanatus var.
citroides (Cucurbitaceae).
The results revealed that CLG was cytotoxic to HT-29.
CLG increased significantly (P<0.05) RNA and protein levels of caspase-3 in HT-29 cells when verified using a colorimetric assay and realtime qPCR, respectively.
The results showed that lipopolysaccharide/interferon-gamma (LPS/INF-γ) increased nitrous oxide (NO) production inR AW264.7macrophages, whereas N(G)-nitro-L-argininemethyl ester (L-NAME) and CLG curtailed it.
This compound did not reveal any cytotoxicity on RAW264.7 macrophages and human normal liver cells (WRL-68) when tested using the MTT assay.
Findings of ferric reducing antioxidant power (FRAP) and oxygen radical absorption capacity (ORAC) assays demonstrate the antioxidant properties of CLG.
The apoptogenic property of CLG on HT-29 cells is thus related to inhibition of reactive nitrogen and oxygen reactive species and the triggering of caspase-3-regulated apoptosis.
American Psychological Association (APA)
Abdel Wahab, Siddig Ibrahim& Hassan, Loiy Elsir Ahmed& Abdul Majid, Amin M. S.& Yagi, Sakina M. Ahmed& Mohan, Syam& Elhassan, Manal Mohamed…[et al.]. 2012. Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1028348
Modern Language Association (MLA)
Abdel Wahab, Siddig Ibrahim…[et al.]. Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-1028348
American Medical Association (AMA)
Abdel Wahab, Siddig Ibrahim& Hassan, Loiy Elsir Ahmed& Abdul Majid, Amin M. S.& Yagi, Sakina M. Ahmed& Mohan, Syam& Elhassan, Manal Mohamed…[et al.]. Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1028348
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028348