Flavonoids Isolated from Korea Citrus aurantium L. Induce G2M Phase Arrest and Apoptosis in Human Gastric Cancer AGS Cells
المؤلفون المشاركون
Lee, Won-Sup
Park, Kwang-Il
Hah, Young-Sool
Chung, Hyon-Jong
An, Su-Jin
Kim, Jin-A.
Kim, Gon-Sup
Park, Hyeon-Soo
Lee, Do Hoon
Kim, Eun-Hee
Nagappan, Arulkumar
Kang, Sang-Rim
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-12-13
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Aim of the Study.
Citrus species is used in traditional medicine as medicinal herb in several Asian countries including Korea.
Flavonioids became known as various properties, such as anti-oxidants, anti-inflammation and anti-cancer, and so forth.
The present study, the anti-cancer effect of flavonioids isolated from Citrus aurantium L.
in human gastric cancer AGS cells has been investigated.
Materials and Methods.
The anti-proliferative activity was assayed using MTT assay.
Cell cycle analysis was done using flow cytometry and apoptosis detection was done using by hoechst fluorescent staining and Annexin V-propidium iodide double staining.
Western blot was used to detect the expression of protein related with cell cycle and apoptosis.
Results.
Flavonoids isolated from Citrus aurantium L.
have the effect of anti proliferation on AGS cells with IC50 value of 99 μg/mL.
Flavonoids inhibited cell cycle progression in the G2/M phase and decrease expression level of cyclin B1, cdc 2, cdc 25c.
Flavonoids induced apoptosis through activate caspase and inactivate PARP.
Conclusions.
Flavonoids isolated from Citrus aurantium L.
induced G2/M phase arrest through the modulation of cell cycle related proteins and apoptosis through activation caspase.
These finding suggest flavonoids isolated from Citrus aurantium L.
were useful agent for the chemoprevention of gastric cancer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lee, Do Hoon& Park, Kwang-Il& Park, Hyeon-Soo& Kang, Sang-Rim& Nagappan, Arulkumar& Kim, Jin-A.…[et al.]. 2011. Flavonoids Isolated from Korea Citrus aurantium L. Induce G2M Phase Arrest and Apoptosis in Human Gastric Cancer AGS Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1028374
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lee, Do Hoon…[et al.]. Flavonoids Isolated from Korea Citrus aurantium L. Induce G2M Phase Arrest and Apoptosis in Human Gastric Cancer AGS Cells. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-1028374
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lee, Do Hoon& Park, Kwang-Il& Park, Hyeon-Soo& Kang, Sang-Rim& Nagappan, Arulkumar& Kim, Jin-A.…[et al.]. Flavonoids Isolated from Korea Citrus aurantium L. Induce G2M Phase Arrest and Apoptosis in Human Gastric Cancer AGS Cells. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1028374
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1028374
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر