Flavonoids Isolated from Korea Citrus aurantium L. Induce G2M Phase Arrest and Apoptosis in Human Gastric Cancer AGS Cells
Joint Authors
Lee, Won-Sup
Park, Kwang-Il
Hah, Young-Sool
Chung, Hyon-Jong
An, Su-Jin
Kim, Jin-A.
Kim, Gon-Sup
Park, Hyeon-Soo
Lee, Do Hoon
Kim, Eun-Hee
Nagappan, Arulkumar
Kang, Sang-Rim
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Aim of the Study.
Citrus species is used in traditional medicine as medicinal herb in several Asian countries including Korea.
Flavonioids became known as various properties, such as anti-oxidants, anti-inflammation and anti-cancer, and so forth.
The present study, the anti-cancer effect of flavonioids isolated from Citrus aurantium L.
in human gastric cancer AGS cells has been investigated.
Materials and Methods.
The anti-proliferative activity was assayed using MTT assay.
Cell cycle analysis was done using flow cytometry and apoptosis detection was done using by hoechst fluorescent staining and Annexin V-propidium iodide double staining.
Western blot was used to detect the expression of protein related with cell cycle and apoptosis.
Results.
Flavonoids isolated from Citrus aurantium L.
have the effect of anti proliferation on AGS cells with IC50 value of 99 μg/mL.
Flavonoids inhibited cell cycle progression in the G2/M phase and decrease expression level of cyclin B1, cdc 2, cdc 25c.
Flavonoids induced apoptosis through activate caspase and inactivate PARP.
Conclusions.
Flavonoids isolated from Citrus aurantium L.
induced G2/M phase arrest through the modulation of cell cycle related proteins and apoptosis through activation caspase.
These finding suggest flavonoids isolated from Citrus aurantium L.
were useful agent for the chemoprevention of gastric cancer.
American Psychological Association (APA)
Lee, Do Hoon& Park, Kwang-Il& Park, Hyeon-Soo& Kang, Sang-Rim& Nagappan, Arulkumar& Kim, Jin-A.…[et al.]. 2011. Flavonoids Isolated from Korea Citrus aurantium L. Induce G2M Phase Arrest and Apoptosis in Human Gastric Cancer AGS Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1028374
Modern Language Association (MLA)
Lee, Do Hoon…[et al.]. Flavonoids Isolated from Korea Citrus aurantium L. Induce G2M Phase Arrest and Apoptosis in Human Gastric Cancer AGS Cells. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-1028374
American Medical Association (AMA)
Lee, Do Hoon& Park, Kwang-Il& Park, Hyeon-Soo& Kang, Sang-Rim& Nagappan, Arulkumar& Kim, Jin-A.…[et al.]. Flavonoids Isolated from Korea Citrus aurantium L. Induce G2M Phase Arrest and Apoptosis in Human Gastric Cancer AGS Cells. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1028374
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028374