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In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable ChitosanNano-HydroxyapatiteCollagen Scaffold
المؤلفون المشاركون
Chen, Yan
Huang, Zhi
Li, Songjian
Zhou, Zhilai
Zhang, Yichen
Feng, Qingling
Li, Xiaoming
Yu, Bo
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-05-28
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
The purpose of this study was to evaluate the in vitro cell biocompatibility of an in situ forming composite consisting of chitosan (CS), nano-hydroxyapatite and collagen (nHAC), which has a complex hierarchical structure similar to natural bone.
MC3T3-E1 mouse calvarial preosteoblasts were cultured on the surface of the injectable CS/nHAC and CS scaffold.
The proliferations of seeded MC3T3-E1 were investigated for 10 days.
Cytotoxicity, cell proliferation, and cell expression of osteogenic markers such as alkaline phosphatase (ALP), type 1 collagen (COL-1), RUNX-2, and osteocalcin (OCN) were examined by biochemical assay and reverse transcription polymerase chain reaction.
Cell viability and total cellularity (measured by dsDNA) were similar between the two scaffold groups.
However, ALP, COL-1, OCN, and RUNX-2 production were significantly greater when osteoblasts were cultured on CS/nHAC scaffolds.
The increase in osteogenic markers production on CS/nHAC scaffolds indicated that these scaffolds were superior to chitosan-only scaffolds in facilitating osteoblast mineralization.
These results demonstrate the potential of the CS/nHAC scaffolds to be used in bone tissue engineering.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Yan& Huang, Zhi& Li, Xiaoming& Li, Songjian& Zhou, Zhilai& Zhang, Yichen…[et al.]. 2012. In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable ChitosanNano-HydroxyapatiteCollagen Scaffold. Journal of Nanomaterials،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-1029205
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Yan…[et al.]. In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable ChitosanNano-HydroxyapatiteCollagen Scaffold. Journal of Nanomaterials No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-1029205
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Yan& Huang, Zhi& Li, Xiaoming& Li, Songjian& Zhou, Zhilai& Zhang, Yichen…[et al.]. In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable ChitosanNano-HydroxyapatiteCollagen Scaffold. Journal of Nanomaterials. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-1029205
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1029205
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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