In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable ChitosanNano-HydroxyapatiteCollagen Scaffold
Joint Authors
Chen, Yan
Huang, Zhi
Li, Songjian
Zhou, Zhilai
Zhang, Yichen
Feng, Qingling
Li, Xiaoming
Yu, Bo
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-28
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The purpose of this study was to evaluate the in vitro cell biocompatibility of an in situ forming composite consisting of chitosan (CS), nano-hydroxyapatite and collagen (nHAC), which has a complex hierarchical structure similar to natural bone.
MC3T3-E1 mouse calvarial preosteoblasts were cultured on the surface of the injectable CS/nHAC and CS scaffold.
The proliferations of seeded MC3T3-E1 were investigated for 10 days.
Cytotoxicity, cell proliferation, and cell expression of osteogenic markers such as alkaline phosphatase (ALP), type 1 collagen (COL-1), RUNX-2, and osteocalcin (OCN) were examined by biochemical assay and reverse transcription polymerase chain reaction.
Cell viability and total cellularity (measured by dsDNA) were similar between the two scaffold groups.
However, ALP, COL-1, OCN, and RUNX-2 production were significantly greater when osteoblasts were cultured on CS/nHAC scaffolds.
The increase in osteogenic markers production on CS/nHAC scaffolds indicated that these scaffolds were superior to chitosan-only scaffolds in facilitating osteoblast mineralization.
These results demonstrate the potential of the CS/nHAC scaffolds to be used in bone tissue engineering.
American Psychological Association (APA)
Chen, Yan& Huang, Zhi& Li, Xiaoming& Li, Songjian& Zhou, Zhilai& Zhang, Yichen…[et al.]. 2012. In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable ChitosanNano-HydroxyapatiteCollagen Scaffold. Journal of Nanomaterials،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-1029205
Modern Language Association (MLA)
Chen, Yan…[et al.]. In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable ChitosanNano-HydroxyapatiteCollagen Scaffold. Journal of Nanomaterials No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-1029205
American Medical Association (AMA)
Chen, Yan& Huang, Zhi& Li, Xiaoming& Li, Songjian& Zhou, Zhilai& Zhang, Yichen…[et al.]. In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable ChitosanNano-HydroxyapatiteCollagen Scaffold. Journal of Nanomaterials. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-1029205
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029205