Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential

المؤلفون المشاركون

Nelson, Brandon
Kan, Natalia G.
Hohenstein Elliott, Kristi A.
Peterson, Cory
Soundararajan, Anuradha
Spiering, Sean
Mercola, Mark
Bright, Gary R.

المصدر

Stem Cells International

العدد

المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-13، 13ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2012-05-30

دولة النشر

مصر

عدد الصفحات

13

الملخص EN

Proper maintenance of stem cells is essential for successful utilization of ESCs/iPSCs as tools in developmental and drug discovery studies and in regenerative medicine.

Standardization is critical for all future applications of stem cells and necessary to fully understand their potential.

This study reports a novel approach for the efficient, consistent expansion of human ESCs and iPSCs using laser sectioning, instead of mechanical devices or enzymes, to divide cultures into defined size clumps for propagation.

Laser-mediated propagation maintained the pluripotency, quality, and genetic stability of ESCs/iPSCs and led to enhanced differentiation potential.

This approach removes the variability associated with ESC/iPSC propagation, significantly reduces the expertise, labor, and time associated with manual passaging techniques and provides the basis for scalable delivery of standardized ESC/iPSC lines.

Adoption of standardized protocols would allow researchers to understand the role of genetics, environment, and/or procedural effects on stem cells and would ensure reproducible production of stem cell cultures for use in clinical/therapeutic applications.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Hohenstein Elliott, Kristi A.& Peterson, Cory& Soundararajan, Anuradha& Kan, Natalia G.& Nelson, Brandon& Spiering, Sean…[et al.]. 2012. Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential. Stem Cells International،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1030052

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Hohenstein Elliott, Kristi A.…[et al.]. Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential. Stem Cells International No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-1030052

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Hohenstein Elliott, Kristi A.& Peterson, Cory& Soundararajan, Anuradha& Kan, Natalia G.& Nelson, Brandon& Spiering, Sean…[et al.]. Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1030052

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1030052