Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential
Joint Authors
Nelson, Brandon
Kan, Natalia G.
Hohenstein Elliott, Kristi A.
Peterson, Cory
Soundararajan, Anuradha
Spiering, Sean
Mercola, Mark
Bright, Gary R.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-30
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Proper maintenance of stem cells is essential for successful utilization of ESCs/iPSCs as tools in developmental and drug discovery studies and in regenerative medicine.
Standardization is critical for all future applications of stem cells and necessary to fully understand their potential.
This study reports a novel approach for the efficient, consistent expansion of human ESCs and iPSCs using laser sectioning, instead of mechanical devices or enzymes, to divide cultures into defined size clumps for propagation.
Laser-mediated propagation maintained the pluripotency, quality, and genetic stability of ESCs/iPSCs and led to enhanced differentiation potential.
This approach removes the variability associated with ESC/iPSC propagation, significantly reduces the expertise, labor, and time associated with manual passaging techniques and provides the basis for scalable delivery of standardized ESC/iPSC lines.
Adoption of standardized protocols would allow researchers to understand the role of genetics, environment, and/or procedural effects on stem cells and would ensure reproducible production of stem cell cultures for use in clinical/therapeutic applications.
American Psychological Association (APA)
Hohenstein Elliott, Kristi A.& Peterson, Cory& Soundararajan, Anuradha& Kan, Natalia G.& Nelson, Brandon& Spiering, Sean…[et al.]. 2012. Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential. Stem Cells International،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1030052
Modern Language Association (MLA)
Hohenstein Elliott, Kristi A.…[et al.]. Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential. Stem Cells International No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-1030052
American Medical Association (AMA)
Hohenstein Elliott, Kristi A.& Peterson, Cory& Soundararajan, Anuradha& Kan, Natalia G.& Nelson, Brandon& Spiering, Sean…[et al.]. Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1030052
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030052