Profiling of Hepatocellular Carcinoma Cell Cycle Regulating Genes Targeted by Calycosin
المؤلفون المشاركون
Wang, Haibao
Wang, Shufang
Zhu, Liguo
Tian, Yaping
Li, Yu
Wang, Deqing
Zhang, Dongqing
Zhuang, Yuan
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-12-23
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
We cocultured calycosin with human hepatocellular carcinoma cell line (BEL-7402) to investigate the effect on cell proliferation.
Calycosin can markedly block the cell growth in G1 phase (P<0.01) on the IC50 concentration.
There were seventeen genes involved in cell-cycle regulation showing differentially expressed in treated cells detected by gene chip.
Eight genes were upregulated and nine genes were downregulated.
Downregulated TFDP-1, CDKN2D, and SPK2 and upregulated CDC2 and CCNB1 might affect cell cycle of tumor cells.
Furthermore, we checked the transcription pattern using 2D gel method to find different expression of proteins in human hepatocellular carcinoma cells after exposure to calycosin.
Fourteen proteins were identified by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS).
Twelve proteins expression were increased such as transgelin 2, pyridoxine 5′-phosphate, stress-induced-phosphoprotein 1, peroxiredoxin 1, endoplasmic reticulum protein 29, and phosphoglycerate mutase 1.
Only thioredoxin peroxidase and high-mobility group box1 proteins’ expression decreased.
Both genes and proteins changes might be relate to the mechanism of antitumor effect under treatment of calycosin.
In conclusion, calycosin has a potential effect to inhibit the BEL-7402 cell growth by inhibiting some oncogene expression and increasing anticancer genes expression, what is more, by blocking cell cycle.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Dongqing& Wang, Shufang& Zhu, Liguo& Tian, Yaping& Wang, Haibao& Zhuang, Yuan…[et al.]. 2013. Profiling of Hepatocellular Carcinoma Cell Cycle Regulating Genes Targeted by Calycosin. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030371
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Dongqing…[et al.]. Profiling of Hepatocellular Carcinoma Cell Cycle Regulating Genes Targeted by Calycosin. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1030371
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Dongqing& Wang, Shufang& Zhu, Liguo& Tian, Yaping& Wang, Haibao& Zhuang, Yuan…[et al.]. Profiling of Hepatocellular Carcinoma Cell Cycle Regulating Genes Targeted by Calycosin. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030371
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1030371
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر