Profiling of Hepatocellular Carcinoma Cell Cycle Regulating Genes Targeted by Calycosin
Joint Authors
Wang, Haibao
Wang, Shufang
Zhu, Liguo
Tian, Yaping
Li, Yu
Wang, Deqing
Zhang, Dongqing
Zhuang, Yuan
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-23
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We cocultured calycosin with human hepatocellular carcinoma cell line (BEL-7402) to investigate the effect on cell proliferation.
Calycosin can markedly block the cell growth in G1 phase (P<0.01) on the IC50 concentration.
There were seventeen genes involved in cell-cycle regulation showing differentially expressed in treated cells detected by gene chip.
Eight genes were upregulated and nine genes were downregulated.
Downregulated TFDP-1, CDKN2D, and SPK2 and upregulated CDC2 and CCNB1 might affect cell cycle of tumor cells.
Furthermore, we checked the transcription pattern using 2D gel method to find different expression of proteins in human hepatocellular carcinoma cells after exposure to calycosin.
Fourteen proteins were identified by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS).
Twelve proteins expression were increased such as transgelin 2, pyridoxine 5′-phosphate, stress-induced-phosphoprotein 1, peroxiredoxin 1, endoplasmic reticulum protein 29, and phosphoglycerate mutase 1.
Only thioredoxin peroxidase and high-mobility group box1 proteins’ expression decreased.
Both genes and proteins changes might be relate to the mechanism of antitumor effect under treatment of calycosin.
In conclusion, calycosin has a potential effect to inhibit the BEL-7402 cell growth by inhibiting some oncogene expression and increasing anticancer genes expression, what is more, by blocking cell cycle.
American Psychological Association (APA)
Zhang, Dongqing& Wang, Shufang& Zhu, Liguo& Tian, Yaping& Wang, Haibao& Zhuang, Yuan…[et al.]. 2013. Profiling of Hepatocellular Carcinoma Cell Cycle Regulating Genes Targeted by Calycosin. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030371
Modern Language Association (MLA)
Zhang, Dongqing…[et al.]. Profiling of Hepatocellular Carcinoma Cell Cycle Regulating Genes Targeted by Calycosin. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1030371
American Medical Association (AMA)
Zhang, Dongqing& Wang, Shufang& Zhu, Liguo& Tian, Yaping& Wang, Haibao& Zhuang, Yuan…[et al.]. Profiling of Hepatocellular Carcinoma Cell Cycle Regulating Genes Targeted by Calycosin. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030371
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030371